Ephrin-B3 Antibody (MM0264- 12X1) - Azide and BSA Free

Immunohistochemistry-Paraffin: Ephrin-B3 Antibody (MM0264- 12X1) [NBP2-12283] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12283. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: MM0264- 12X1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Ephrin-B3 Antibody (MM0264- 12X1) - Azide and BSA Free Summary

• Immunogen: Human recombinant Ephrin-B3 (extracellular domain)
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Mouse
• Gene: EFNB3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Ephrin-B3 Antibody (MM0264- 12X1) - Azide and BSA Free

• EFL-6
• Efnb3
• Elk-L3
• EPH-related receptor tyrosine kinase ligand 8
• Ephrin B3
• EphrinB3
• Ephrin-B3
• Epl8
• EPLG8EPH-related receptor transmembrane ligand ELK-L3
• LERK-8
• LERK8EFL6
• NLERK-2

Background

Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EFNB3
• Uniprot:
  • Q15768()