Ephrin-B3 Antibody (88838)

• Reactivity: Hu
• Applications: WB
• Clone: 88838
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Ephrin-B3 Antibody (88838) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Ephrin-B3
  Leu28-Ser224
  Accession # Q15768
• Specificity: Detects human Ephrin-B3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, rmEphrin-A2, recombinant human (rh) Ephrin-A3, rhEphrin-A4, rhEphrin-A5, rmEphrin-B1, and rmEphrin-B2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EFNB3
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ephrin-B3 Antibody (88838)

• EFL-6
• Efnb3
• Elk-L3
• EPH-related receptor tyrosine kinase ligand 8
• Ephrin B3
• EphrinB3
• Ephrin-B3
• Epl8
• EPLG8EPH-related receptor transmembrane ligand ELK-L3
• LERK-8
• LERK8EFL6
• NLERK-2

Background

Ephrin-B3, also known as NLERK-2, Elk-L3, EFL-6, ELF-3 and LERK-8 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can be tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 aa long and are highly conserved, especially the last 33 aa. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B3 has been shown to bind Ephrin-A4, Ephrin-B1, Ephrin-B2, and Ephrin-B3 (2, 3). The extracellular domains of murine and human Ephrin-B3 share 98% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

1. Eph Nomenclature Committee [letter]. Cell (1997) 90:403.
2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ephrin-B3 Antibody (MAB395)(1)

Publication using MAB395

• N Vilallongu, J Schaeffer, AM Hesse, C Delpech, B Blot, A Paccard, E Plissonnie, B Excoffier, Y Couté, S Belin, H Nawabi Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system Nature Communications, 2022-10-13;13(1):6040. 2022-10-13 [PMID: 36229455] (IHC, Mouse)

Review for Ephrin-B3 Antibody (MAB395) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 04/04/2020

Summary

• Application: Western Blot
• Sample Tested: BT-20 human breast cancer cell line,ZR-75 human breast cancer cell line
• Species: Human
• Lot: EQG031805A

Bioinformatics

• Gene Symbol: EFNB3
• Uniprot:
  • Q15768()