EphB3 Antibody [Unconjugated]

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Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) followed by HRP-conjugated ...read more
EphB3 was detected in immersion fixed COLO 205 human colorectal adeno­carcinoma cell line using Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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EphB3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB3
Leu30-Thr537
Accession # P54754
Specificity
Detects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human EphB3 is observed and less than 1% cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphA4, rmEphB2, rmEphB4, and rmEphB6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHB3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF432 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB3 Antibody [Unconjugated]

  • Cek10
  • EC 2.7.10
  • EC 2.7.10.1
  • EK2
  • EPH receptor B3
  • EphB3
  • EPH-like kinase 2
  • EPH-like tyrosine kinase-2
  • ephrin type-B receptor 3
  • ETK2Hek2
  • Hek2
  • human embryo kinase 2
  • Mdk5
  • Sek4
  • Tyro6
  • Tyrosine-protein kinase TYRO6

Background

EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 525 amino acid (aa) extracellular domain (ECD) of mature mouse EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, mouse EphB3 shares 96% and 99% aa sequence identity with human and rat EphB3, respectively. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6‑9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10‑12). It directs embyronic neuronal axon pathfinding, and its upregulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10‑12, 15).
  1. Pasquale, E.B. (2008) Cell 133:38.
  2. Ruiz, J.C. et al. (1994) Mech. Dev. 48:153.
  3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
  4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
  5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
  6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
  7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
  8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
  9. Cortina, C. et al. (2007) Nature Genet. 39:1376.
  10. Birgbauer, E. et al. (2000) Development 127:1231.
  11. Alfaro, D. et al. (2008) Immunology 125:131.
  12. Risley, M. et al. (2009) Mech. Dev. 126:230.
  13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
  14. Batlle, E. et al. (2005) Nature 435:1126.
  15. Holmberg, J. et al. (2006) Cell 125:1151.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EphB3 Antibody (AF432)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Flow Cytometry, IF, IHC, IHC-Fr, IHC-P, Western Blot.


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Flow Cytometry
(1)
IF
(1)
IHC
(4)
IHC-Fr
(2)
IHC-P
(2)
Western Blot
(2)
All Applications
Filter By Species
Mouse
(10)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF432 Applications Species
AE Lewis, A Kuwahara, J Franzosi, JO Bush Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 2022-03-15;38(11):110510. 2022-03-15 [PMID: 35294885] (IF, Mouse) IF Mouse
L Farr, S Ghosh, N Jiang, K Watanabe, M Parlak, R Bucala, S Moonah CD74 signaling links inflammation to intestinal epithelial cell regeneration and promotes mucosal healing Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):. 2020-01-28 [PMID: 32004754] (IHC, Mouse) IHC Mouse
AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation J. Cell Biol., 2016-10-17;215(2):217-229. 2016-10-17 [PMID: 27810913] (IHC, Mouse) IHC Mouse
Omanma Adighibe, Russell D. Leek, Marta Fernandez-Mercado, Jiangting Hu, Cameron Snell, Kevin C. Gatter et al. Why some tumours trigger neovascularisation and others don’t: the story thus far Chinese Journal of Cancer 2016-12-01 [PMID: 26873439]
Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium. PLoS Genet, 2015-11-03;11(11):e1005638. 2015-11-03 [PMID: 26528816] (IHC-P, Mouse) IHC-P Mouse
Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa Aberrant EphB/ephrin-B expression in experimental gastric lesions and tumor cells World Journal of Gastroenterology 2015-01-14 [PMID: 25593460]
Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013-12-12;504(7479):277-81. 2013-12-12 [PMID: 24336287] (IHC-Fr, Mouse) IHC-Fr Mouse
Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression. Biochem. Biophys. Res. Commun., 2011-01-26;405(4):521-6. 2011-01-26 [PMID: 21276420] (IHC-P, Western Blot, Mouse) IHC-P, Western Blot Mouse
Yoshitatsu Sei, Xinping Lu, Alice Liou, Xilin Zhao, Stephen A. Wank A stem cell marker-expressing subset of enteroendocrine cells resides at the crypt base in the small intestine American Journal of Physiology-Gastrointestinal and Liver Physiology 2011-02-01 [PMID: 21088235]
Bush JO, Soriano P Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism. Genes Dev., 2009-06-10;23(13):1586-99. 2009-06-10 [PMID: 19515977] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 13 Publications.

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Bioinformatics

Gene Symbol EPHB3
Uniprot