EphA1 Antibody

Western Blot: EphA1 Antibody [NBP2-16345] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA1 antibody [C1C3-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: EphA1 Antibody [NBP2-16345] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EphA1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human EphA1. The exact sequence is proprietary.
• Predicted Species: Rat (96%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPHA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 108 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for EphA1 Antibody

• EC 2.7.10
• EC 2.7.10.1
• EPH receptor A1
• eph tyrosine kinase 1
• EphA1
• EPHephrin type-A receptor 1
• EPHT
• EPHT1erythropoietin-producing hepatoma amplified sequence
• Esk
• MGC163163
• oncogene EPH
• soluble EPHA1 variant 1
• soluble EPHA1 variant 2
• Tyrosine-protein kinase receptor EPH

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EPHA1
• Uniprot:
  • P21709()