EphA5 Antibody (86731) [Unconjugated]

Images

 
EphA5 was detected in immersion fixed U-118-MG human glioblastoma/astrocytoma cell line using 10 µg/mL Mouse Anti-Human/Rat EphA5 Monoclonal Antibody (Catalog # MAB541) for 3 hours at room temperature. Cells were ...read more
U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human/Rat EphA5 Monoclonal Antibody (Catalog # MAB541, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC
Clone
86731
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

EphA5 Antibody (86731) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat EphA5
Ser58-Gln573 (Asp170Glu)
Accession # P54757
Specificity
Detects rat EphA5 in direct ELISAs and Western blots. In direct ELISAs, 5‑15% cross reactivity with recombinant mouse (rm) EphA4 and rmEpha8 is observed and no cross-reactivity with recombinant human (rh) EphA1, rmEphA2, -A3, -A6, -A7, recombinant rat EphB1, -B2, or rmEphB3 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPHA5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using
MAB541 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA5 Antibody (86731) [Unconjugated]

  • Bsk
  • Cek7
  • EC 2.7.10
  • EC 2.7.10.1
  • Ehk1
  • EHK-1
  • EHK1Hek7
  • EK7
  • EPH homology kinase 1
  • Eph homology kinase-1
  • EPH receptor A5
  • EphA5
  • EPH-like kinase 7
  • ephrin type-A receptor 5
  • Hek7
  • receptor protein-tyrosine kinase HEK7
  • Rek7
  • TYRO4HEK7CEK7
  • tyrosine-protein kinase receptor EHK-1

Background

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA5 has been shown to bind ephrin-A5, ephrin-A1,
ephrin‑A2, ephrin-A3, and ephrin-A4 (2, 3). The extracellular domains of rat EphA5 share 98.5% amino acid identity with the mouse homolog and 96.5% identity with the human homolog. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphA5 Antibody (MAB541)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC.


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ICC
(1)
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(1)
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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EPHA5
Entrez
Uniprot