EphA5 was detected in immersion fixed U-118-MG human glioblastoma/astrocytoma cell line using 10 µg/mL Mouse Anti-Human/Rat EphA5 Monoclonal Antibody (Catalog # MAB541) for 3 hours at room temperature. Cells were ...read more
U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human/Rat EphA5 Monoclonal Antibody (Catalog # MAB541, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more
Mouse myeloma cell line NS0-derived recombinant rat EphA5 Ser58-Gln573 (Asp170Glu) Accession # P54757
Detects rat EphA5 in direct ELISAs and Western blots. In direct ELISAs, 5‑15% cross reactivity with recombinant mouse (rm) EphA4 and rmEpha8 is observed and no cross-reactivity with recombinant human (rh) EphA1, rmEphA2, -A3, -A6, -A7, recombinant rat EphB1, -B2, or rmEphB3 is observed.
Protein A or G purified from hybridoma culture supernatant
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for EphA5 Antibody (86731) [Unconjugated]
EPH homology kinase 1
Eph homology kinase-1
EPH receptor A5
EPH-like kinase 7
ephrin type-A receptor 5
receptor protein-tyrosine kinase HEK7
tyrosine-protein kinase receptor EHK-1
EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA5 has been shown to bind ephrin-A5, ephrin-A1, ephrin‑A2, ephrin-A3, and ephrin-A4 (2, 3). The extracellular domains of rat EphA5 share 98.5% amino acid identity with the mouse homolog and 96.5% identity with the human homolog. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
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