Recombinant Mouse EphA7 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse EphA7 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphA7 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant human Ephrin-A4 Fc Chimera with a linear range of 0.16 ‑ 10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse EphA7 protein
Mouse EphA7
(Ala30-Pro549)
Accession # JC5673
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala30
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Epha7
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
85.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110 kDa, reducing conditions
Publications
Read Publications using
608-A7 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EphA7 Fc Chimera Protein, CF

  • Cek11
  • Ebk
  • EC 2.7.10
  • EC 2.7.10.1
  • Ehk3
  • EHK-3
  • EK11
  • EPH homology kinase 3
  • Eph homology kinase-3
  • EPH receptor A7
  • EphA7
  • EPH-like kinase 11
  • ephrin type-A receptor 7
  • Hek11
  • Mdk1
  • receptor protein-tyrosine kinase HEK11
  • tyrosine-protein kinase receptor EHK-3

Background

EphA7, also known as Mdk1, Hek11, Ehk3, Ebk, and Cek11, is a 115-120 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases (1, 2). The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA7 preferentially binds to and is activated by Ephrin-A1, -A2, -A3, -A4, and -A5 (3 - 6). Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. The 527 amino acid (aa) extracellular domain (ECD) of mouse EphA7 contains an N‑terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type III domains (FnIII). The 421 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (7). Within the ECD, mouse EphA7 shares 98% and 99% aa sequence identity with human and rat EphA7, respectively. Alternate splicing generates a differentially expressed kinase-defective isoform of mouse EphA7 that lack most of the cytoplasmic domain and a soluble isoform that is truncated following the first FnIII domain (4, 6 - 9). In mouse, EphA7 is expressed in discrete regions of the developing and adult neocortex (4, 7 - 10), Purkinje layer of the cerebellum (7), limbic system (4, 8 ‑ 10), visual and auditory systems (7, 8, 11), and the peripheral sensory nervous system (7, 8). EphA7 functions as a repulsive guidance molecule during the targeting of retinal axons to the superior colliculus  and of neocortical axons to the thalamus (4, 10, 11). EphA7 is also expressed in mesenchymal cells along routes of axon innervation during limb development (12). EphA7 ligands are expressed in a complementary pattern during embryogenesis (3, 10 - 12). EphA7 is selectively expressed in early stages of the B cell lineage, and a 50 kDa secreted form is produced by mature peripheral B and T cells (6, 13). This isoform is also expressed in human lung carcinoma (14). EphA7 can be up or downregulated in a variety of human cancers (6, 15, 16).
  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Ciossek, T. and A. Ullrich (1997) Oncogene 14:35.
  4. Miller, K. et al. (2006) J. Comp. Neurol. 496:627.
  5. Gale, N.W. et al. (1996) Neuron 17:9.
  6. Dawson, D.W. et al. (2007) Oncogene 26:4243.
  7. Ciossek, T. et al. (1995) Oncogene 9:97.
  8. Mori, T. et al. (1995) Mol. Brain Res. 34:154.
  9. Ciossek, T. et al. (1999) Mol. Brain Res. 74:231.
  10. Torii, M. and P. Levitt (2005) Neuron 48:563.
  11. Rashid, T. et al. (2005) Neuron 47:57.
  12. Araujo, M. et al. (1998) Development 125:4195.
  13. Aasheim, H.-C. et al. (1997) Blood 90:3613.
  14. Tsuboi, M. et al. (2010) Int. J. Oncol. 36:635.
  15. Hafner, C. et al. (2004) Clin. Chem. 3:490.
  16. Wang, J. et al. (2007) Hum. Pathol. 38:1649.

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Publications for EphA7 (608-A7)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 6 applications: Binding Assay, Bioassay, ICC, IHC, IHC-Fr, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(10)
ICC
(1)
IHC
(1)
IHC-Fr
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(11)
Rat
(1)
Chicken
(3)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 608-A7 Applications Species
LL Arnold, A Cecchini, DA Stark, J Ihnat, RN Craigg, A Carter, S Zino, D Cornelison EphA7 promotes myogenic differentiation via cell-cell contact Elife, 2020;9(0):. 2020 [PMID: 32314958] (Bioassay, Mouse) Bioassay Mouse
S Li, J Zhai, J Liu, F Di, Y Sun, W Li, ZJ Chen, Y Du Erythropoietin-producing hepatocellular A7 triggering ovulation indicates a potential beneficial role for polycystic ovary syndrome EBioMedicine, 2018;0(0):. 2018 [PMID: 30292674] (In Vivo, Mouse) In Vivo Mouse
Weiss A, Airik R, Bohnenpoll T, Greulich F, Foik A, Trowe M, Rudat C, Costantini F, Adams R, Kispert A Nephric duct insertion requires EphA4/EphA7 signaling from the pericloacal mesenchyme. Development, 2014;141(17):3420-30. 2014 [PMID: 25139858] (Bioassay, Mouse) Bioassay Mouse
Defourny J, Poirrier A, Lallemend F, Mateo Sanchez S, Neef J, Vanderhaeghen P, Soriano E, Peuckert C, Kullander K, Fritzsch B, Nguyen L, Moonen G, Moser T, Malgrange B Ephrin-A5/EphA4 signalling controls specific afferent targeting to cochlear hair cells. Nat Commun, 2013;4(0):1438. 2013 [PMID: 23385583] (Bioassay, Mouse) Bioassay Mouse
Park , E, Kim , Y, Noh , H, Lee , H, Yoo , S, Park , S EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development. Cell Death Differ, 2013;20(1):169-80. 2013 [PMID: 22976838] (Bioassay, Mouse) Bioassay Mouse
Dewannieux M, Vernochet C, Ribet D, Bartosch B, Cosset FL, Heidmann T The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins. PLoS Pathog., 2011;7(10):e1002309. 2011 [PMID: 22028653] (IHC-Fr, Mouse) IHC-Fr Mouse
Marler KJ, Poopalasundaram S, Broom ER Pro-neurotrophins secreted from retinal ganglion cell axons are necessary for ephrinA-p75NTR-mediated axon guidance. Neural Dev, 2010;5(0):30. 2010 [PMID: 21044296] (Bioassay, Chicken) Bioassay Chicken
Genander M, Holmberg J, Frisen J Ephrins negatively regulate cell proliferation in the epidermis and hair follicle. Stem Cells, 2010;28(7):1196-205. 2010 [PMID: 20506314] (In Vivo, Mouse) In Vivo Mouse
Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010;30(12):4246-55. 2010 [PMID: 20335460] (Bioassay, Rat) Bioassay Rat
Torii M, Hashimoto-Torii K, Levitt P, Rakic P Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling. Nature, 2009;461(7263):524-8. 2009 [PMID: 19759535] (IHC, Mouse) IHC Mouse
Show All 16 Publications.

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Bioinformatics

Gene Symbol Epha7
Uniprot