EphA1 Antibody (5D2)

Western Blot: EphA1 Antibody (5D2D1) [NB100-1763] - Analysis using EphA1 mouse mAb against A549 (1) and Hela (2) cell lysate.

Immunohistochemistry-Paraffin: EphA1 Antibody (5D2D1) [NB100-1763] - Analysis of human ovary carcinoma (left) and breast carcinoma (right), showing cytoplasmic localization using EphA1 mouse mAb with DAB staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IHC-P
• Clone: 5D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EphA1 Antibody (5D2) Summary

• Immunogen: Purified recombinant fragment of EphA1 expressed in E. Coli.
• Specificity: EPH Receptor A1 (5D2D1)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPHA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
• Theoretical MW: 108 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for EphA1 Antibody (5D2)

• EC 2.7.10
• EC 2.7.10.1
• EPH receptor A1
• eph tyrosine kinase 1
• EphA1
• EPHephrin type-A receptor 1
• EPHT
• EPHT1erythropoietin-producing hepatoma amplified sequence
• Esk
• MGC163163
• oncogene EPH
• soluble EPHA1 variant 1
• soluble EPHA1 variant 2
• Tyrosine-protein kinase receptor EPH

Background

EPH receptor A1 (EphA1), with 976-amino acid protein(about 107 kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date and their ligands, the ephrins,can be subdivided into two major subclasses, ephrin-A and ephrin-B. Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior, particularly in the nervous system. They have been implicated in various developmental processes, including axonal guidance, angiogenesis, morphogenesis and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPHA1
• Entrez:
  • 2041(Human)