Home » EphA1 » EphA1 Antibodies » EphA1 Antibody (043)

EphA1 Antibody (043)

 Submit a Review

Images

 
There are currently no images for EphA1 Antibody (NBP2-90732).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Clone
043
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-90732AF350
Alexa Fluor 405
NBP2-90732AF405
Alexa Fluor 488
NBP2-90732AF488
Alexa Fluor 532
NBP2-90732AF532
Alexa Fluor 594
NBP2-90732AF594
Alexa Fluor 647
NBP2-90732AF647
Alexa Fluor 700
NBP2-90732AF700
Alexa Fluor 750
NBP2-90732AF750
Biotin
NBP2-90732B
DyLight 350
NBP2-90732UV
DyLight 405
NBP2-90732V
DyLight 488
NBP2-90732G
DyLight 550
NBP2-90732R
DyLight 594
NBP2-90732DL594
DyLight 650
NBP2-90732C
DyLight 680
NBP2-90732FR
DyLight 755
NBP2-90732IR
FITC
NBP2-90732F
HRP
NBP2-90732H
100% Guarantee on every product

EphA1 Antibody (043) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EphA1 (Q60750; Met1-Glu548).
Specificity
No cross-reactivity in ELISA with:

Mouse EphA6
Mouse EPHA7
Mouse EPHB3
Mouse EPHB4
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
EPHA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for EphA1 Antibody (043)

  • EC 2.7.10
  • EC 2.7.10.1
  • EPH receptor A1
  • eph tyrosine kinase 1
  • EphA1
  • EPHephrin type-A receptor 1
  • EPHT
  • EPHT1erythropoietin-producing hepatoma amplified sequence
  • Esk
  • MGC163163
  • oncogene EPH
  • soluble EPHA1 variant 1
  • soluble EPHA1 variant 2
  • Tyrosine-protein kinase receptor EPH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Ephrin-B1
473-EB
Ephrin-B1
(1 Review)
(24 Publications)
Species: Mu
Applications: Bind
Ephrin-B2
[Unconjugated]
496-EB
Ephrin-B2 [Unconjugated]
(1 Review)
(39 Publications)
Species: Mu
Applications: Bind
EphA4 Antibody
AF641
EphA4 was detected in immersion fixed frozen sections of embryonic rat rib cartilage primordium (E15) using 5 µg/mL Goat Anti-Mouse EphA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF641) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
(8 Publications)
Species: Mu
Applications: IHC, WB
EphA2 Antibody
[Unconjugated]
AF3035
Western blot shows lysates of A431 human epithelial carcinoma parental cell line and EphA2 knock out (KO) A431 cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EphA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3035) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EphA2 at approximately 110 kDa (as indicated), but not detectable in the knockout A431 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EphA2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human EphA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3035) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EphA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3035, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
(10 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, KO, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(9 Reviews)
(37 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Ephrin-A1
602-A1
Ephrin-A1
(1 Review)
(19 Publications)
Species: Mu
Applications: Bind
Ephrin-A2
8415-A2
Ephrin-A2
Species: Mu
Applications: BA
Ephrin-A5
374-EA
Ephrin-A5
(1 Review)
(31 Publications)
Species: Hu
Applications: Bind
EphB4 Antibody
AF446
EphB4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse EphB4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF446) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(4 Reviews)
(31 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
EphB1
[Unconjugated]
1596-B1
EphB1 [Unconjugated]
(8 Publications)
Species: Rt
Applications: Bind
EphA3
640-A3
EphA3
(6 Publications)
Species: Mu
Applications: Bind
EphB3 Antibody
[Unconjugated]
AF432
EphB3 was detected in immersion fixed COLO 205 human colorectal adeno­carcinoma cell line using Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EphB3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(10 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, WB
Ephrin-B3 Antibody
[Unconjugated]
AF395
Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Ephrin‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF395) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Ephrin‑B3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Publications)
Species: Hu
Applications: IHC, WB
EphA7
[Unconjugated]
608-A7
EphA7 [Unconjugated]
(16 Publications)
Species: Mu
Applications: Bind
Ephrin-A3
359-EA
Ephrin-A3
(10 Publications)
Species: Hu
Applications: Bind
EphA5 Antibody
(86731) [Unconjugated
MAB541
EphA5 was detected in immersion fixed U-118-MG human glioblastoma/astrocytoma cell line using 10 µg/mL Mouse Anti-Human/Rat EphA5 Monoclonal Antibody (Catalog # MAB541) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human/Rat EphA5 Monoclonal Antibody (Catalog # MAB541, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
(1 Publication)
Species: Hu, Rt
Applications: CyTOF-ready, Flow, ICC, WB
EphB6 Antibody
AF611
Western blot shows lysates of mouse thymus tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF611) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB6 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(6 Publications)
Species: Mu
Applications: WB
Src [p Tyr419]
Antibody [Unconjugate
AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Review)
(6 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
VEGF [HRP]
DVE00
(26 Reviews)
(588 Publications)
Species: Hu
Applications: ELISA
Ephrin-A4 Antibody
[Unconjugated]
AF369
Ephrin‑A4 was detected in immersion fixed paraffin-embedded sections of normal human mammary glands using Goat Anti-Human Ephrin‑A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF369) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane of glandular epithelium. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Ephrin‑A4 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Ephrin‑A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF369) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(2 Publications)
Species: Hu
Applications: ICC, IHC, WB

Publications for EphA1 Antibody (NBP2-90732) (0)

There are no publications for EphA1 Antibody (NBP2-90732).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EphA1 Antibody (NBP2-90732) (0)

There are no reviews for EphA1 Antibody (NBP2-90732). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for EphA1 Antibody (NBP2-90732) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional EphA1 Products

Bioinformatics Tool for EphA1 Antibody (NBP2-90732)

Discover related pathways, diseases and genes to EphA1 Antibody (NBP2-90732). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EphA1 Antibody (NBP2-90732)

Discover more about diseases related to EphA1 Antibody (NBP2-90732).
 

Pathways for EphA1 Antibody (NBP2-90732)

View related products by pathway.

PTMs for EphA1 Antibody (NBP2-90732)

Learn more about PTMs related to EphA1 Antibody (NBP2-90732).
 

Research Areas for EphA1 Antibody (NBP2-90732)

Find related products by research area.

Blogs on EphA1

There are no specific blogs for EphA1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EphA1 Antibody (043) and receive a gift card or discount.


Bioinformatics

Gene Symbol EPHA1