Novus Biologicals products are now on bio-techne.com

EpCAM/TROP1 Products

Antibodies
EpCAM/TROP1 Antibody [Unconju ...
EpCAM/TROP1 Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Host: Goat Polyclonal
Conjugate Catalog # Availability Size Price
Formulation Catalog # Availability Price  
EpCAM/TROP1 Antibody (158210) ...
EpCAM/TROP1 Antibody (158210) [Unc...
MAB960
Species: Hu
Applications: WB, IHC, ICC/IF
Host: Mouse Monoclonal
Formulation Catalog # Availability Price  
Antibody Pairs
EpCAM/TROP1 Antibody Pair [HR ...
EpCAM/TROP1 Antibody Pair [HRP]
NBP2-79378
Species: Mu
Applications: ELISA
Host: Rabbit Monoclonal
Conjugate Catalog # Availability Size Price
EpCAM/TROP1 Antibody Pair [HR ...
EpCAM/TROP1 Antibody Pair [HRP]
NBP2-79512
Species: Hu
Applications: ELISA
Host: Monoclonal
Conjugate Catalog # Availability Size Price
ELISA Kits
Human EpCAM/TROP1 DuoSet ELIS ...
Human EpCAM/TROP1 DuoSet ELISA, 15...
DY960
Species: Hu
Applications: ELISA
Mouse EpCAM/TROP1 ELISA Kit ( ...
Mouse EpCAM/TROP1 ELISA Kit (Color...
NBP3-00429
Species: Mu
Applications: ELISA
Lysates
EpCAM/TROP1 293T Cell Transie ...
EpCAM/TROP1 293T Cell Transient Ov...
H00004072-T02
Species: Hu
Applications: WB
Proteins
Recombinant Human EpCAM/TROP1 ...
Recombinant Human EpCAM/TROP1 Fc C...
960-EP
Species: Hu
Applications: Bioactivity
Formulation Catalog # Availability Price  
Recombinant Human EpCAM/TROP1 ...
Recombinant Human EpCAM/TROP1 His-...
9277-EP
Species: Hu
Applications: Bioactivity
Formulation Catalog # Availability Price  
Recombinant Human EpCAM/TROP1 ...
Recombinant Human EpCAM/TROP1 Fc C...
AVI960
Species: Hu
Applications: Bioactivity
Conjugate Catalog # Availability Size Price
Formulation Catalog # Availability Price  

Description

Epithelial cell adhesion molecule (EpCAM), also known as TROP1 and CD326, is a type I transmembrane glycoprotein that plays a role in a number of cellular processes including adhesion, signaling, maintaining stemness, proliferation, migration, and invasion (1,2). The human EpCAM protein is 314 amino acids (aa) in length with a theoretical molecular weight (MW) of ~35 kDa (1,3,4). The EpCAM protein includes a signal peptide, an extracellular N-terminal domain, a thyroglobulin type-1 domain, a carboxyl-terminal domain, a single-pass transmembrane domain, and an intracellular domain (1,3). The protein is highly conserved and has approximately 81% aa sequence identity between human and mouse (5). Trophoblast cell-surface antigen 2 (TROP2) also shares ~67% aa sequence similarity with EpCAM (1,5). While both EpCAM and TROP2 are cell surface markers expressed in the epithelium, their expression levels typically do not correlate (5). EpCAM is expressed in normal epithelial tissue and elevated expression is observed in many epithelial carcinomas (1-3) Patient tumor samples with increased EpCAM expression have been associated with poor prognosis (1). Given EpCAM's elevated expression in tumors, it has become a potential target for cancer therapy approaches, including monoclonal antibody treatment and anti-EpCAM trispecific antibodies (1,5).

EpCAM functions as an intracellular signaling molecule and contributes to regulation of epithelial-to-mesenchymal (EMT) transition (4,5). EpCAM is cleaved during the process of regulated intramembrane proteolysis (RIP) (4,5). Initial cleavage occurs at the membrane via a disintegrin and metalloprotease 17 (ADAM17) which releases EpCAM's extracellular domain (EpEx) (4,5). A secondary cleavage is mediated by presenilin 2 (PSEN2) which releases EpCAM's cytoplasmic trail (EpICD) (4,5). EpICD translocates to the nucleus where it associates with beta-catenin, FHL-2, and LEF-1 and induces transcription of genes related to EMT and tumor growth (4,5). EpCAM has also been shown to regulate structure and functionality of the apical junction complex of cells through direct interaction with claudin-7 and association with E-cadherin (2,5). Loss of EpCAM disrupts adherins junction and tight junction structure and function (2).

References

1. Mohtar MA, Syafruddin SE, Nasir SN, Low TY. Revisiting the Roles of Pro-Metastatic EpCAM in Cancer. Biomolecules. 2020; 10(2):255. https://doi.org/10.3390/biom10020255

2. Huang L, Yang Y, Yang F, et al. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018; 42(4):1771-1785. https://doi.org/10.3892/ijmm.2018.3764

3. Brown TC, Sankpal NV, Gillanders WE. Functional Implications of the Dynamic Regulation of EpCAM during Epithelial-to-Mesenchymal Transition. Biomolecules. 2021; 11(7):956. https://doi.org/10.3390/biom11070956

4. Uniprot (P16422)

5. Schnell U, Cirulli V, Giepmans BN. EpCAM: structure and function in health and disease. Biochim Biophys Acta. 2013; 1828(8):1989-2001. https://doi.org/10.1016/j.bbamem.2013.04.018

Bioinformatics

Entrez Mouse
Rat
Human
Uniprot Human
Human
Human
Human
Product By Gene ID 4072
Alternate Names
  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2EGP34
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Research Areas for EpCAM/TROP1

Find related products by research area and learn more about each of the different research areas below.

Cancer

Related EpCAM/TROP1 Blog Posts

Check out the latest blog posts on EpCAM/TROP1.
Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...    Read more.
Read more EpCAM/TROP1 related blogs.