EpCAM/TROP1 Antibody (AUA1) - BSA Free

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Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer ...read more
Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Detection of EpCAM/CD326 in two Human mammary tumor cell lines, MDA-MB-231 (mesenchyme-like) and MCF-7 (epithelium-like). Dilution: 1:500 in PBS with 5% BSA. ...read more
Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The ...read more
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue with 1:500 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica ...read more
Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Using the PE direct conjugate A cell surface stain was performed on HT-29 cells with EpCAM (AUA1) antibody NB600-1182PE (blue) and a matched isotype control ...read more
Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of A431 lysate (A) and Hek293 lysate (B) using EpCAM/CD326 antibody (AUA1) at 2 ug/ml. Non-reduced lysate was used in each lane.
Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using EpCAM/CD326 antibody (blue) and matched Isotype control (orange). An ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
AUA1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

EpCAM/TROP1 Antibody (AUA1) - BSA Free Summary

Immunogen
LoVo cell line preparation (Human).
Localization
Cell Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPCAM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Use in immunofluorescence reported in scientific literature (PMID: 21763624).

This antibody is CyTOF ready.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB600-1182 in the following applications:

Publications
Read Publications using
NB600-1182 in the following applications:

  • 1 publication
  • 1 publication
  • 3 publications
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for EpCAM/TROP1 Antibody (AUA1) - BSA Free

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Background

Epithelial cell adhesion molecule (EpCAM), also known as TROP1 and CD326, is a type I transmembrane glycoprotein that plays a role in a number of cellular processes including adhesion, signaling, maintaining stemness, proliferation, migration, and invasion (1,2). The human EpCAM protein is 314 amino acids (aa) in length with a theoretical molecular weight (MW) of ~35 kDa (1,3,4). The EpCAM protein includes a signal peptide, an extracellular N-terminal domain, a thyroglobulin type-1 domain, a carboxyl-terminal domain, a single-pass transmembrane domain, and an intracellular domain (1,3). The protein is highly conserved and has approximately 81% aa sequence identity between human and mouse (5). Trophoblast cell-surface antigen 2 (TROP2) also shares ~67% aa sequence similarity with EpCAM (1,5). While both EpCAM and TROP2 are cell surface markers expressed in the epithelium, their expression levels typically do not correlate (5). EpCAM is expressed in normal epithelial tissue and elevated expression is observed in many epithelial carcinomas (1-3) Patient tumor samples with increased EpCAM expression have been associated with poor prognosis (1). Given EpCAM's elevated expression in tumors, it has become a potential target for cancer therapy approaches, including monoclonal antibody treatment and anti-EpCAM trispecific antibodies (1,5).

EpCAM functions as an intracellular signaling molecule and contributes to regulation of epithelial-to-mesenchymal (EMT) transition (4,5). EpCAM is cleaved during the process of regulated intramembrane proteolysis (RIP) (4,5). Initial cleavage occurs at the membrane via a disintegrin and metalloprotease 17 (ADAM17) which releases EpCAM's extracellular domain (EpEx) (4,5). A secondary cleavage is mediated by presenilin 2 (PSEN2) which releases EpCAM's cytoplasmic trail (EpICD) (4,5). EpICD translocates to the nucleus where it associates with beta-catenin, FHL-2, and LEF-1 and induces transcription of genes related to EMT and tumor growth (4,5). EpCAM has also been shown to regulate structure and functionality of the apical junction complex of cells through direct interaction with claudin-7 and association with E-cadherin (2,5). Loss of EpCAM disrupts adherins junction and tight junction structure and function (2).

References

1. Mohtar MA, Syafruddin SE, Nasir SN, Low TY. Revisiting the Roles of Pro-Metastatic EpCAM in Cancer. Biomolecules. 2020; 10(2):255. https://doi.org/10.3390/biom10020255

2. Huang L, Yang Y, Yang F, et al. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018; 42(4):1771-1785. https://doi.org/10.3892/ijmm.2018.3764

3. Brown TC, Sankpal NV, Gillanders WE. Functional Implications of the Dynamic Regulation of EpCAM during Epithelial-to-Mesenchymal Transition. Biomolecules. 2021; 11(7):956. https://doi.org/10.3390/biom11070956

4. Uniprot (P16422)

5. Schnell U, Cirulli V, Giepmans BN. EpCAM: structure and function in health and disease. Biochim Biophys Acta. 2013; 1828(8):1989-2001. https://doi.org/10.1016/j.bbamem.2013.04.018

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EpCAM/TROP1 Antibody (NB600-1182)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: FLOW, Flow, ICC/IF, IHC, WB.


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FLOW
(1)
Flow
(1)
ICC/IF
(3)
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Showing Publications 1 - 9 of 9.
Publications using NB600-1182 Applications Species
Chang ZM, Wang Z, Shao D et al. Shape Engineering Boosts Magnetic Mesoporous Silica Nanoparticle-Based Isolation and Detection of Circulating Tumor Cells. ACS Appl Mater Interfaces 2018 Mar 20 [PMID: 29468874] (Flow)

Details:
This citation used the PE version of this antibody.
Flow
Albarenque SM, Zwacka RM, Mohr A. Both human and mouse mesenchymal stem cells promote breast cancer metastasis Stem Cell Res 2011 Sep [PMID: 21763624]
Aleksic T, Chitnis MM, Perestenko OV et al. Type 1 insulin-like growth factor receptor translocates to the nucleus of human tumor cells. Cancer Res. 2010 Aug 15 [PMID: 20710042] (WB, Human) WB Human
Ntouroupi TG, Ashraf SQ, Mcgregor SB et al. Detection of circulating tumour cells in peripheral blood with an automated scanning fluorescence microscope. Br J Cancer. 2008 Sep 2 [PMID: 18682708] (ICC/IF, Human) ICC/IF Human
Conaghan P, Ashraf S, Tytherleigh M et al. Targeted killing of colorectal cancer cell lines by a humanised IgG1 monoclonal antibody that binds to membrane-bound carcinoembryonic antigen. Br J Cancer. 2008 Apr 8 [PMID: 18349843] (FLOW, Human) FLOW Human
El-Hariry I, Pignatelli M, Lemoine NR et al. FGF-1 and FGF-2 modulate the E-cadherin/catenin system in pancreatic adenocarcinoma cell lines. Br J Cancer. 2001 Jun 15 [PMID: 11401320]
Jordinson M, El-Hariry I, Calnan D et al. Vicia faba agglutinin, the lectin present in broad beans, stimulates differentiation of undifferentiated colon cancer cells. Gut. 1999 May [PMID: 10205210] (WB, Human) WB Human
Anagnostaki E, Skaarlos D, Tamvakis N et al. Immunohistochemical and immunocytochemical study of bladder carcinomas using the epithelium-specific, tumour-associated monoclonal antibodies HMFG1 and AUA1. Br J Cancer Suppl. 1990 Jul [PMID: 2200496] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Kocjan G, Sweeney E, Miller KD, Bobrow L et al. AUA1: new immunocytochemical marker for detecting epithelial cells in body cavity fluids. J Clin Pathol. 1992 Apr [PMID: 1577978] (ICC/IF, Human) ICC/IF Human

Review for EpCAM/TROP1 Antibody (NB600-1182) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-1182:
Filter by Applications
Western Blot
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot EpCAM/TROP1 NB600-1182
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5
reviewed by:
Fabio Anzanello
Western Blot Human 03/06/2017
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Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
LotA-3

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EpCAM/TROP1 Antibody (NB600-1182). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for EpCAM/TROP1 Antibody (NB600-1182)

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Blogs on EpCAM/TROP1.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

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Recent Reviews

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Fabio Anzanello
03/06/2017
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol EPCAM
Entrez