EpCAM/TROP1 Antibody (AUA1)

Images

 
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody ...read more
Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Western blot analysis of A431 lysate (A) and Hek293 lysate (B) using EpCAM/CD326 antibody (AUA1) at 2 ug/ml. Non-reduced lysate was used in each lane.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue with 1:500 dilution of EpCAM/TROP1 antibody (clone AUA1) ...read more
Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Using the PE direct conjugate A cell surface stain was performed on HT-29 cells with EpCAM (AUA1) antibody NB600-1182PE (blue) and a matched isotype control ...read more
Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using EpCAM/CD326 antibody (dark blue). Isotype control shown in orange. An ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
AUA1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

EpCAM/TROP1 Antibody (AUA1) Summary

Immunogen
LoVo cell line preparation (Human).
Localization
Cell Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPCAM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • CyTOF-ready
Application Notes
Immunofluorescence (PMID: 21763624) Use in western blot reported in scientific literature ( PMID 10205210) Use in Flow Cytometry reported in scientific literature (PMID 18349843) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB600-1182 in the following applications:

Publications
Read Publications using
NB600-1182 in the following applications:

  • 1 publication
  • 3 publications
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for EpCAM/TROP1 Antibody (AUA1)

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Background

Cross reacts with breast carcinomas, kidney tubules, tonsil crypt epithelium and cells in some serious effusions. Useful in characterisation of all epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EpCAM/TROP1 Antibody (NB600-1182)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, WB.


Filter By Application
FLOW
(1)
ICC/IF
(3)
IHC
(1)
WB
(2)
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Filter By Species
Human
(6)
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Showing Publications 1 - 8 of 8.
Publications using NB600-1182 Applications Species
Albarenque SM, Zwacka RM, Mohr A. Both human and mouse mesenchymal stem cells promote breast cancer metastasis Stem Cell Res 2011 Sep [PMID: 21763624]
Aleksic T, Chitnis MM, Perestenko OV et al. Type 1 insulin-like growth factor receptor translocates to the nucleus of human tumor cells. Cancer Res. 2010 Aug 15 [PMID: 20710042] (WB, Human) WB Human
Ntouroupi TG, Ashraf SQ, Mcgregor SB et al. Detection of circulating tumour cells in peripheral blood with an automated scanning fluorescence microscope. Br J Cancer. 2008 Sep 2 [PMID: 18682708] (ICC/IF, Human) ICC/IF Human
Conaghan P, Ashraf S, Tytherleigh M et al. Targeted killing of colorectal cancer cell lines by a humanised IgG1 monoclonal antibody that binds to membrane-bound carcinoembryonic antigen. Br J Cancer. 2008 Apr 8 [PMID: 18349843] (FLOW, Human) FLOW Human
El-Hariry I, Pignatelli M, Lemoine NR et al. FGF-1 and FGF-2 modulate the E-cadherin/catenin system in pancreatic adenocarcinoma cell lines. Br J Cancer. 2001 Jun 15 [PMID: 11401320]
Jordinson M, El-Hariry I, Calnan D et al. Vicia faba agglutinin, the lectin present in broad beans, stimulates differentiation of undifferentiated colon cancer cells. Gut. 1999 May [PMID: 10205210] (WB, Human) WB Human
Anagnostaki E, Skaarlos D, Tamvakis N et al. Immunohistochemical and immunocytochemical study of bladder carcinomas using the epithelium-specific, tumour-associated monoclonal antibodies HMFG1 and AUA1. Br J Cancer Suppl. 1990 Jul [PMID: 2200496] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Kocjan G, Sweeney E, Miller KD, Bobrow L et al. AUA1: new immunocytochemical marker for detecting epithelial cells in body cavity fluids. J Clin Pathol. 1992 Apr [PMID: 1577978] (ICC/IF, Human) ICC/IF Human

Review for EpCAM/TROP1 Antibody (NB600-1182) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB600-1182:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot EpCAM/TROP1 NB600-1182
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reviewed by:
Anonymous
WB Human 03/06/2017
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Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
LotA-3

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EpCAM/TROP1 Antibody (NB600-1182). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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1
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Anonymous
03/06/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol EPCAM
Entrez