ENPP-3/CD203c Antibody (NP4D6)

Images

 
Immunocytochemistry/ Immunofluorescence: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, ...read more
Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human peripheral blood by anti-CD203c antibody (NP4D6) FITC.
Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human basophils in allergen-stimulated whole blood (CD203cpos/ SSClow) by anti-CD203c antibody (NP4D6)
Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human basophils in IgE-activated whole blood by anti-CD203c antibody (NP4D6) APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, CyTOF-ready
Clone
NP4D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ENPP-3/CD203c Antibody (NP4D6) Summary

Immunogen
HEK-293 cells transfected with human CD203c
Specificity
The mouse monoclonal antibody NP4D6 reacts with CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation. HLDA VIII
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ENPP3
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1 - 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for ENPP-3/CD203c Antibody (NP4D6)

  • B10
  • CD203c antigen
  • CD203c
  • dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3)
  • dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3)
  • ectonucleotide pyrophosphatase/phosphodiesterase 3
  • ectonucleotide pyrophosphatase/phosphodiesterase family member 3
  • E-NPP 3
  • ENPP3
  • ENPP-3
  • gp130RB13-6
  • NPP3
  • PD-IBETA
  • PDNP3
  • PDNP3B10
  • Phosphodiesterase I beta
  • Phosphodiesterase I/nucleotide pyrophosphatase 3
  • phosphodiesterase-I beta

Background

CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for ENPP-3/CD203c Antibody (NBP1-44643) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-44643AF350
Alexa Fluor 405 NBP1-44643AF405
Alexa Fluor 488 NBP1-44643AF488
Alexa Fluor 532 NBP1-44643AF532
Alexa Fluor 594 NBP1-44643AF594
Alexa Fluor 647 NBP1-44643AF647
Alexa Fluor 700 NBP1-44643AF700
Alexa Fluor 750 NBP1-44643AF750
Allophycocyanin NBP1-44643APC
Allophycocyanin/Cy7 NBP1-44643APCCY7
DyLight 350 NBP1-44643UV
DyLight 405 NBP1-44643V
DyLight 488 NBP1-44643G
DyLight 550 NBP1-44643R
DyLight 594 NBP1-44643DL594
DyLight 650 NBP1-44643C
DyLight 680 NBP1-44643FR
DyLight 755 NBP1-44643IR
Janelia Fluor 549 NBP1-44643JF549
Janelia Fluor 646 NBP1-44643JF646
PE NBP1-44643PE
PE/Atto594 NBP1-44643PEATT594
PE/Cy5.5 NBP1-44643PECY55
PE/Cy7 NBP1-44643PECY7
PerCP NBP1-44643PCP

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