Endophilin B1/Bif-1 Antibody

Images

 
Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.
Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Mk, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.57 mg/ml

Order Details

Endophilin B1/Bif-1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Bif-1. The exact sequence is proprietary.
Localization
Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Mitochondrion outer membrane, Peripheral membrane protein
Predicted Species
Rat (100%), Porcine (100%), Xenopus (100%), Zebrafish (100%), Monkey (100%), Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SH3GLB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-31130 in the following applications:

  • WB
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (100%), Xenopus Tropicalis (100%), Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.57 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Endophilin B1/Bif-1 Antibody

  • Bax-interacting factor 1
  • Bif1
  • Bif-1
  • Bif-1dJ612B15.2
  • CGI-61
  • Endophilin B1
  • endophilin-B1
  • KIAA0491endophilin B1
  • SH3 domain-containing GRB2-like protein B1
  • SH3-containing protein SH3GLB1
  • SH3-domain GRB2-like endophilin B1
  • SH3-domain, GRB2-like, endophilin B1
  • SH3GLB1

Background

Bif, a member of the endophilin B protein family may be an important component of the mitochondrial pathway for apoptosis, as a novel Bax/Bak activator, and loss of this proapoptotic molecule may contribute to tumorigenesis. Endophilins have been proposed to play roles in membrane trafficking reactions through interactions with endocytic protein via physical or chemical modifications of the membrane bilayer, particularly in presynaptic vesicle trafficking at nerve terminals. Bif (Endophilin B1), like endophilins A, binds to palmitoyl CoA, and exhibits lysophosphatidic acid acyl transferase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endophilin B1/Bif-1 Antibody (NBP1-31130)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SH3GLB1
Entrez
Uniprot