Endophilin B1/Bif-1 Antibody - BSA Free

Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Endophilin B1/Bif-1 antibody [C2C3], C-term detects Bif-1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.Green: Bif-1 protein stained by Bif-1 antibody [C2C3], C-term (NBP1-31130).Blue: Hoechst 33342 staining.Scale bar = 10 um

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bif-1 antibody (NBP1-31130).Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Endophilin B1/Bif-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Endophilin B1/Bif-1 Antibody - BSA Free (NBP1-31130) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Endophilin B1/Bif-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Endophilin B1/Bif-1. The exact sequence is proprietary.
• Localization: Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Mitochondrion outer membrane, Peripheral membrane protein
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Zebrafish (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SH3GLB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (100%), Xenopus Tropicalis (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Endophilin B1/Bif-1 Antibody - BSA Free

• Bax-interacting factor 1
• Bif1
• Bif-1
• Bif-1dJ612B15.2
• CGI-61
• Endophilin B1
• endophilin-B1
• KIAA0491endophilin B1
• SH3 domain-containing GRB2-like protein B1
• SH3-containing protein SH3GLB1
• SH3-domain GRB2-like endophilin B1
• SH3-domain, GRB2-like, endophilin B1
• SH3GLB1

Background

Bif, a member of the endophilin B protein family may be an important component of the mitochondrial pathway for apoptosis, as a novel Bax/Bak activator, and loss of this proapoptotic molecule may contribute to tumorigenesis. Endophilins have been proposed to play roles in membrane trafficking reactions through interactions with endocytic protein via physical or chemical modifications of the membrane bilayer, particularly in presynaptic vesicle trafficking at nerve terminals. Bif (Endophilin B1), like endophilins A, binds to palmitoyl CoA, and exhibits lysophosphatidic acid acyl transferase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Endophilin B1/Bif-1 Antibody (NBP1-31130)(1)

Publication using NBP1-31130

• Mir SU, George NM, Zahoor L et al. Inhibition of Autophagic Turnover in b-cells by Fatty acids and Glucose leads to Apoptotic cell Death. J. Biol. Chem. 2014-12-29 [PMID: 25548282] (WB, Human)

Bioinformatics

• Gene Symbol: SH3GLB1
• Entrez:
  • 51100(Human)
• Uniprot:
  • Q9Y371()