Endoglin/CD105 Antibody [Unconjugated]

Images

 
Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 15 µg/mL overnight at 4 ...read more
Western blot shows lysates of human kidney tissue, human lung tissue, human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of ...read more
Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 1.7 µg/mL overnight ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for ...read more
Endoglin/CD105 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Endoglin/CD105 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105
Glu26-Gly586
Accession # Q5T9B9
Specificity
Detects human Endoglin in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse Endoglin and recombinant rat Endoglin is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ENG
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 20 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
Control
Endoglin/CD105 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
AF1097 in the following applications:

Publications
Read Publications using
AF1097 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endoglin/CD105 Antibody [Unconjugated]

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and Betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human endoglin cDNA encodes 658 amino acids including a 25 amino acid (aa) signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine, and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14).  In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFlt-1), along with low Placental Growth Factor (PlGF), are pathogenic due to antiangiogenic activity (15).

  1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endoglin/CD105 Antibody (AF1097)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(4)
IHC-P
(6)
Western Blot
(2)
All Applications
Filter By Species
Human
(13)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF1097 Applications Species
Q Zhang, DY Yi, BZ Xue, WW Wen, YP Lu, A Abdelmakso, MX Sun, DT Yuan, HY Zhao, NX Xiong, W Xiang, P Fu CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression Cell Death Dis, 2018;9(11):1101. 2018 [PMID: 30368520] (IHC, Human) IHC Human
HF Moffett, ME Coon, S Radtke, SB Stephan, L McKnight, A Lambert, BL Stoddard, HP Kiem, MT Stephan Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers Nat Commun, 2017;8(1):389. 2017 [PMID: 28855514] (Flow Cytometry, Human) Flow Cytometry Human
ML García-Her, NO Uribe-Urib, R Espinosa-G, WM Kast, SA Khader, J Rangel-Mor A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression Front Immunol, 2017;8(0):563. 2017 [PMID: 28567040] (IHC-P, Human) IHC-P Human
Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling Nat. Cell Biol., 2017;0(0):. 2017 [PMID: 28530660] (IHC, Mouse) IHC Mouse
Evaluation of Nonperfused Retinal Vessels in Ischemic Retinopathy Invest Ophthalmol Vis Sci, 2016;57(11):5031-5037. 2016 [PMID: 27661854] (IHC-P, Human) IHC-P Human
Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease Sci Rep, 2016;6(0):32382. 2016 [PMID: 27660926] (IHC, Human) IHC Human
Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension PLoS ONE, 2016;11(9):e0162144. 2016 [PMID: 27589391] (IHC-P, Human) IHC-P Human
Riordan D, Varma S, West R, Brown P Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 2015;10(7):e0128975. 2015 [PMID: 26176839] (IHC-P, Human) IHC-P Human
Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients. PLoS ONE, 2015;10(3):e0121421. 2015 [PMID: 25803686] (IHC-P, Human) IHC-P Human
Kim J, Piao Y, Pak Y, Chung D, Han Y, Hong J, Jun E, Shim J, Choi J, Kim C Umbilical cord mesenchymal stromal cells affected by gestational diabetes mellitus display premature aging and mitochondrial dysfunction. Stem Cells Dev, 2015;24(5):575-86. 2015 [PMID: 25437179] (Western Blot, Human) Western Blot Human
Show All 14 Publications.

Review for Endoglin/CD105 Antibody (AF1097) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1097:
Filter by Applications
IF
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23623496
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23623496

Details

Detection NotesSee PMID 23623496

Comments

CommentsPublished PMID 23623496

Product General Protocols

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WB Video Protocol

FAQs for Endoglin/CD105 Antibody (AF1097). (Showing 1 - 2 of 2 FAQs).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1097

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Anonymous
01/05/2015
Application: IF
Species: Human

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