Endoglin/CD105 Antibody (209701) [Fluorescein]

MS-1 mouse pancreatic islet endothelial cell line was stained with Rat Anti-Mouse Endoglin/CD105 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1320F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 209701
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

Endoglin/CD105 Antibody (209701) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105
  Glu21-Gly581 (predicted)
  Accession # NP_031958
• Specificity: Detects mouse Endoglin/CD105 in direct ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant human Endoglin/CD105.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ENG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endoglin/CD105 Antibody (209701) [Fluorescein]

• CD105 antigen
• CD105
• Endoglin
• ENDOsler-Rendu-Weber syndrome 1
• ENG
• HHT1FLJ41744
• ORW
• ORW1

Background

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the Zona Pellucida (ZP) family of proteins (1-3). Endoglin and Betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal, and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-Endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with Activin-A and BMP-7 via Activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP-7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5 but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low Placental Growth Factor (PlGF), are pathogenic due to anti-angiogenic activity (15).

1. Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endoglin/CD105 Antibody (FAB1320F)(5)

Publications using FAB1320F

• Latifa Mohamed Abdelgawad, Manar Mohy Abd El-hamed, Dina Sabry, Marwa Abdelgwad, Department of Medical Laser applications, National Institute of laser enhanced science, Cairo University, Egypt., Department of Medical Laser application, National Institute of laser enhanced science, Cairo University, Egypt et al. Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Keap1/Nrf2/Ho-1 Pathway Reports of Biochemistry and Molecular Biology 4/1/2021 [PMID: 34277866]
• Latifa Mohamed Abdelgawad, Asmaa Mohamed Abdelaziz, Dina Sabry, Marwa Abdelgwad Influence of photobiomodulation and vitamin D on osteoblastic differentiation of human periodontal ligament stem cells and bone-like tissue formation through enzymatic activity and gene expression Biomolecular Concepts 12/23/2020 [PMID: 34233429]
• Madhu V, Li C, Dighe A, Balian G, Cui Q BMP-non-responsive Sca1+ CD73+ CD44+ mouse bone marrow derived osteoprogenitor cells respond to combination of VEGF and BMP-6 to display enhanced osteoblastic differentiation and ectopic bone formation. PLoS ONE, 2014-07-21;9(0):. 2014-07-21 [PMID: 25048464] (Flow Cytometry, Mouse)
• Katebi M, Soleimani M, Cronstein BN Adenosine A2A receptors play an active role in mouse bone marrow-derived mesenchymal stem cell development. J. Leukoc. Biol., 2008-12-12;85(3):438-44. 2008-12-12 [PMID: 19056861] (Flow Cytometry, Mouse)
• Sarojini H, Estrada R, Lu H, Dekova S, Lee MJ, Gray RD, Wang E PEDF from mouse mesenchymal stem cell secretome attracts fibroblasts. J. Cell. Biochem., 2008-08-01;104(5):1793-802. 2008-08-01 [PMID: 18348263] (Flow Cytometry, Mouse)

FAQs for Endoglin/CD105 Antibody (FAB1320F). (Showing 1 - 2 of 2 FAQs).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: ENG
• Uniprot:
  • NP_031958()