EMSY Antibody

Images

 
Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit ...read more
Immunohistochemistry: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.
Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human breast carcinoma.
Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human adenocarcinoma.
Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Human prostate carcinoma.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

EMSY Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1250 and the C-terminus (residue 1322) of human EMSY chromosome 11 open reading frame 30 using the numbering given in TrEMBL entry Q8TE50 (GeneID 56946).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
C11ORF30
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Positive Control
HeLa Lysate (NB800-PC9)
Publications
Read Publication using NB100-545.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EMSY Antibody

  • chromosome 11 open reading frame 30
  • EMSYFLJ90741
  • protein EMSY

Background

EMSY binds BRCA2 within a region (exon 3) deleted in cancer. EMSY is capable of silencing the activation potential of BRCA2 exon 3, associates with chromatin regulators HP1 and BS69, and localizes to sites of repair following DNA damage. Its levels are amplified in breast and ovarian cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EMSY Antibody (NB100-545)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional EMSY Products

Bioinformatics Tool for EMSY Antibody (NB100-545)

Discover related pathways, diseases and genes to EMSY Antibody (NB100-545). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for EMSY Antibody (NB100-545)

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Bioinformatics

Gene Symbol C11ORF30
Entrez
Uniprot