NIF1 Antibody

Images

 
Western Blot: NIF1 Antibody [NB100-2579] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. NB100-2579 used for WB at 0.04 ug/ml ...read more
Immunohistochemistry: NIF1 Antibody [NB100-2579] - Sample: FFPE section of human Hodgkin's lymphoma. Antibody: Affinity purified rabbit anti- NIF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

NIF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1342) of human NRC-Interacting Factor 1 using the numbering given in entry NP_071378.1 (GeneID 63925).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZNF335
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
Control
HeLa Lysate (NB800-PC1)
Publications
Read Publication using NB100-2579.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NIF1 Antibody

  • DKFZp586G1122
  • Hzf
  • HZFzinc finger protein 385
  • RZFHematopoietic zinc finger protein
  • ZFP385
  • zinc finger protein 385A
  • ZNF385Retinal zinc finger protein

Background

NIF1 enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. This protein may function by altering local chromatin structure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NIF1 Antibody (NB100-2579)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZNF335
Entrez
Uniprot