NIF1 Antibody

Western Blot: NIF1 Antibody [NB100-2579] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. NB100-2579 used for WB at 0.04 ug/ml (A) and at 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibody BL2737 (NB100-2579) and BL2738. Detection was by chemiluminescence, with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry-Paraffin: NIF1 Antibody [NB100-2579] - Section of human Hodgkin's lymphoma. Antibody: Affinity purified rabbit anti- NIF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NIF1 Antibody Summary

• Description: Novus Biologicals Rabbit NIF1 Antibody (NB100-2579) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-NIF1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1342) of human NRC-Interacting Factor 1 using the numbering given in entry NP_071378.1 (GeneID 63925).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF335
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 -1:500
  • Immunohistochemistry-Paraffin 1:100- 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NIF1 Antibody

• DKFZp586G1122
• Hzf
• HZFzinc finger protein 385
• RZFHematopoietic zinc finger protein
• ZFP385
• zinc finger protein 385A
• ZNF385Retinal zinc finger protein

Background

NIF1 enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. This protein may function by altering local chromatin structure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NIF1 Antibody (NB100-2579)(3)

Publications using NB100-2579

• Yang B, Li W, Wang X et al. Zinc finger protein Zfp335 is required for T cell homeostatic proliferation through regulating Lmnb1 Cell & Bioscience 2025-10-14 [PMID: 41088342]
• Wang X, Jiao A, Sun L et al. Zinc finger protein Zfp335 controls early T-cell development and survival through beta-selection-dependent and -independent mechanisms eLife 2022-02-03 [PMID: 35113015] (Mouse)
• Garapaty S, Mahajan MA, Samuels HH et al. Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1. J Biol Chem 2008-03-01 [PMID: 18180299]

Bioinformatics

• Gene Symbol: ZNF335
• Entrez:
  • 63925(Human)
  • 329559(Mouse)
• Uniprot:
  • NP_071378.1(Human)