ZMYND11 Antibody

Knockdown Validated: ZMYND11 Antibody [NBP2-20960] - Immunoblots of indicated proteins in VCaP human prostate cancer cells overexpressing the indicated SPOP species and in VCaP cancer cells overexpressing the indicated SPOP species with and without ZMYND11 knockdown using two different short-hairpin RNAs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33531470) licensed under a CC-BY licence.

Western Blot: ZMYND11 Antibody [NBP2-20960] - Western Blot analysis of ZMYND11 expression in transfected 293T cell line by ZMYND11 polyclonal antibody. A: Non-transfected lysate. B: ZMYND11 transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ZMYND11 Antibody [NBP2-20960] - Detection of ZMYND11 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZMYND11 protein stained by BS69 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: ZMYND11 Antibody [NBP2-20960] - Human colon carcinoma, using BS69 antibody antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ZMYND11 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human BS69. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (97%), Rat (97%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZMYND11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for ZMYND11 Antibody

• adenovirus 5 E1A binding protein
• Adenovirus 5 E1A-binding protein
• bone morphogenetic protein receptor-associated molecule 1
• BRAM1
• MGC111056
• MYND domain containing 11
• Protein BS69
• zinc finger MYND domain-containing protein 11
• zinc finger, MYND-type containing 11

Background

The protein encoded by this gene was first identified by its ability to bind the adenovirus E1A protein. The protein localizes to the nucleus. It functions as a transcriptional repressor, and expression of E1A inhibits this repression. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ZMYND11 Antibody (NBP2-20960)(1)

Publication using NBP2-20960

• Bernasocchi, T, El Tekle, G Et al. Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer. Nat Commun 2021-02-02 [PMID: 33531470] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: ZMYND11
• Uniprot:
  • Q15326(Human)