EML4 Antibody

Images

 
Western Blot: EML4 Antibody [NBP1-05974] - Whole cell lysate from HeLa cells. EML4 was also immunoprecipitated by rabbit anti-EML4 antibody NBP1-05973.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05974] - Human ovarian carcinoma. Antibody used at a dilution of1:1,000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

EML4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 931 and 981of human echinoderm microtubule associated protein like 4 using the numbering given in entry NP_061936.2 (GeneID 27436).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EML4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Positive Control
EML4 Lysate (NBL1-10258)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EML4 Antibody

  • C2orf2
  • echinoderm microtubule associated protein like 4
  • echinoderm microtubule-associated protein-like 4
  • ELP120
  • EMAP-4
  • EMAPL4
  • FLJ10942
  • FLJ32318
  • Restrictedly overexpressed proliferation-associated protein
  • Ropp 120
  • ROPP120DKFZp686P18118

Background

Echinoderm microtubule associated protein like 4 (EML4) has been identified as part of a gene fusion with the kinase domain of the anaplastic lymphoma receptor tyrosine kinase (ALK) in non-small cell lung cancer. EML4 associates with in vitro polymerized microtubules. In the cell, phosphorylated EML4 associates with the mitotic spindle and is essential for proliferation and microtubule network formation. Alternative names for EML4 include EMAPL4, ropp120, C2orf2, ELP120, and EMAP-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EML4
Entrez
Uniprot