Kinesin 5B Antibody

Images

 
Western Blot: Kinesin 5B Antibody [NBP2-21667] - Western Blot of Kinesin 5B antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 ug of Mouse Brain lysate. Lane 3: 10 ug of Mouse Brain lysate. Primary ...read more
Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667] - Immunohistochemistry of Kinesin 5B antibody. Tissue: Human Brain at 40X at pH 6.
Western Blot: Kinesin 5B Antibody [NBP2-21667] - Glucocorticoid promoted microtubule destabilization and following transport impairment. The hippocampus of mice exposed to rapamycin (8 mg/kg) for 2 days before ...read more
Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667] - 40X in human brain at pH 6. Tissue: Human Brain.
SDS-Page: Kinesin 5B Antibody [NBP2-21667] - SDS-PAGE of Kinesin 5B antibody. Lane 1: Truncated Kinesin-1 Protein. Lane 2: none.Load: 5 ug per lane.Block: coomassie blue for 20 min, destain overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, PAGE
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Kinesin 5B Antibody Summary

Immunogen
Kinesin 5B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human Kinesin 5B protein. (Uniprot: P33176)
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
KIF5B
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • SDS-Page
  • Western Blot 1:100-1:500
Application Notes
For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user.

Use in ICC/IF was reported in scientific literature (PMID: 30429451).
Theoretical MW
109.68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-21667 in the following applications:

Reactivity Notes

Cross-reactivity with Kinesin 5Bfrom other sources have not been determined

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Notes

The product was prepared from monospecific antiserum by delipidation and defibrination

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Kinesin 5B Antibody

  • Conventional kinesin heavy chain
  • kinesin family member 5B
  • kinesin-1 heavy chain
  • KNS1kinesin 1 (110-120kD)
  • KNSKINH
  • Ubiquitous kinesin heavy chain
  • UKHCkinesin heavy chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kinesin 5B Antibody (NBP2-21667)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KIF5B
Uniprot