EMI1 Antibody (EMI1/1176) - IHC-Prediluted

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - IHC-Prediluted [NBP2-48221] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Mouse Monoclonal Antibody (EMI1/1176).

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - IHC-Prediluted [NBP2-48221] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Mouse Monoclonal Antibody (EMI1/1176).

Western Blot of HeLa, HepG2, HEK293 and K562 cell lysates with EMI1 Antibody (EMI1/1176) - IHC-Prediluted.

Analysis of Protein Array containing more than 19,000 full-length human proteins using EMI1 Antibody (EMI1/1176) - IHC-Prediluted. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

• Reactivity: Hu
• Applications: IHC
• Clone: EMI1/1176
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

EMI1 Antibody (EMI1/1176) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)
• Localization: Nuclear
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBXO5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for EMI1 Antibody (EMI1/1176) - IHC-Prediluted

• EMI1Early mitotic inhibitor 1
• F-box only protein 5
• F-box protein 5
• FBX5F-box protein Fbx5
• Fbxo31

Background

Fbp5A is a member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to Xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase-promoting complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBXO5