F-box protein 43 Antibody

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Western Blot: F-box protein 43 Antibody [NB100-2455] - Antibody at 0.3ug/ml. Mouse ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Mu, Rt, Hu, Po, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

F-box protein 43 Antibody Summary

Immunogen
Peptide with sequence C-PGSAQSKRNLKRL corresponding to C-Terminus according to NP_001025031.2.
Predicted Species
Human (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FBXO43
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Flow Cytometry 5 - 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 - 10 ug/ml
Application Notes
Flow cytometry successful usage on rat meiotic testis cells is reported in scientific literature (PMID: 19802389). WB successful usage on rat pachytene spermatocytes showing a 71 kDa band is reported in scientific literature (PMID: 19802389) and in mouse oocytes is reported in scientific literature (PMID: 22302686). This antibody has been successfully used in WB in human (PMID:27605379).
Control
293 Whole Cell Lysate
Publications
Read Publications using
NB100-2455 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 19802389).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004).[supplied by OMIM]

Alternate Names for F-box protein 43 Antibody

  • early mitotic inhibitor 2
  • EMI2Fbx43
  • Endogenous meiotic inhibitor 2
  • ERP1
  • F-box only protein 43
  • F-box protein 43
  • FBX43

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for F-box protein 43 Antibody (NB100-2455)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: FLOW, WB.


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FLOW
(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol FBXO43
Entrez
Uniprot