EMI1 Antibody (EMI1/1176) - Azide and BSA Free

Images

 
There are currently no images for EMI1 Antibody (NBP2-47786).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-P, CyTOF-ready, ICC/IF
Clone
EMI1/1176
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

EMI1 Antibody (EMI1/1176) - Azide and BSA Free Summary

Immunogen
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
Localization
Nuclear
Specificity
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FBXO5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot : 1 - 2 ug/ml
  • Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml
  • CyTOF-ready
  • Immunofluorescence : 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 6q25.2 Western Blotting (1-2ug/ml for 60 minutes at RT); Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for EMI1 Antibody (EMI1/1176) - Azide and BSA Free

  • EMI1Early mitotic inhibitor 1
  • F-box only protein 5
  • F-box protein 5
  • FBX5F-box protein Fbx5
  • Fbxo31

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-66987
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-547
Species: Hu
Applications: WB, ICC/IF, IP
MAB7675
Species: Hu
Applications: WB, Simple Western, ICC
NBP2-15840
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-85729
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00051053-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-31473
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-59828
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-37507
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NB100-2567
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-42388
Species: Hu
Applications: WB, IHC, IHC-P, KD
NBP2-33466
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-38092
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24695
Species: Hu
Applications: WB, IHC-P
NBP2-47786
Species: Hu
Applications: WB, Flow, IHC-P, CyTOF-ready, ICC/IF

Publications for EMI1 Antibody (NBP2-47786) (0)

There are no publications for EMI1 Antibody (NBP2-47786).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EMI1 Antibody (NBP2-47786) (0)

There are no reviews for EMI1 Antibody (NBP2-47786). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EMI1 Antibody (NBP2-47786) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for EMI1 Antibody (NBP2-47786)

Discover related pathways, diseases and genes to EMI1 Antibody (NBP2-47786). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EMI1 Antibody (NBP2-47786)

Discover more about diseases related to EMI1 Antibody (NBP2-47786).
 

Pathways for EMI1 Antibody (NBP2-47786)

View related products by pathway.

PTMs for EMI1 Antibody (NBP2-47786)

Learn more about PTMs related to EMI1 Antibody (NBP2-47786).

Blogs on EMI1

There are no specific blogs for EMI1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EMI1 Antibody (EMI1/1176) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol FBXO5