EMI1 Antibody (EMI1/1176) [DyLight 650]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
EMI1/1176
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

EMI1 Antibody (EMI1/1176) [DyLight 650] Summary

Immunogen
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
Localization
Nuclear
Specificity
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FBXO5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EMI1 Antibody (EMI1/1176) [DyLight 650]

  • EMI1Early mitotic inhibitor 1
  • F-box only protein 5
  • F-box protein 5
  • FBX5F-box protein Fbx5
  • Fbxo31

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for EMI1 Antibody (NBP2-47786C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47786AF350
Alexa Fluor 405 NBP2-47786AF405
Alexa Fluor 488 NBP2-47786AF488
Alexa Fluor 532 NBP2-47786AF532
Alexa Fluor 594 NBP2-47786AF594
Alexa Fluor 647 NBP2-47786AF647
Alexa Fluor 700 NBP2-47786AF700
Alexa Fluor 750 NBP2-47786AF750
Allophycocyanin NBP2-47786APC
Biotin NBP2-47786B
DyLight 350 NBP2-47786UV
DyLight 405 NBP2-47786V
DyLight 488 NBP2-47786G
DyLight 550 NBP2-47786R
DyLight 594 NBP2-47786DL594
DyLight 650 NBP2-47786C
DyLight 680 NBP2-47786FR
DyLight 755 NBP2-47786IR
FITC NBP2-47786F
HRP NBP2-47786H
Janelia Fluor 549 NBP2-47786JF549
Janelia Fluor 646 NBP2-47786JF646
PE NBP2-47786PE
PerCP NBP2-47786PCP

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Bioinformatics Tool for EMI1 Antibody (NBP2-47786C)

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Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

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Bioinformatics

Gene Symbol FBXO5
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