eIF5A Antibody (5E1) - BSA Free

Immunohistochemistry: eIF5A Antibody (5E1) [NBP3-26604] - Image of eIF5A Antibody (5E1) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: eIF5A Antibody (5E1) [NBP3-26604] - Positive Western Blot detected in: A2780 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate. All lanes: eIF5A Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 17, 21 kDa Observed band size: 18 kDa

Immunocytochemistry/Immunofluorescence: eIF5A Antibody (5E1) [NBP3-26604] - Staining of Hela Cells with eIF5A Antibody (5E1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: eIF5A Antibody (5E1) [NBP3-26604] - Overlay histogram showing Jurkat cells stained with eIF5A Antibody (5E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 5E1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

eIF5A Antibody (5E1) - BSA Free Summary

• Description: Novus Biologicals Rabbit eIF5A Antibody (5E1) - BSA Free (NBP3-26604) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human eIF5A [UniProt P63241]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF5A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:20-1:200
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for eIF5A Antibody (5E1) - BSA Free

• eIF4D
• eIF-4D
• eIF5A
• eIF-5A
• EIF5A1
• eIF-5A1
• eIF-5A-1
• eIF5AI
• Eukaryotic initiation factor 5A isoform 1
• eukaryotic initiation factor 5A
• eukaryotic translation initiation factor 5A
• eukaryotic translation initiation factor 5A-1
• MGC104255
• MGC99547
• Rev-binding Factor

Background

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5A