EIF5 Antibody (6D6H11) - BSA Free

Western Blot: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis using EIF5 mAb against human EIF5 (AA: 1-300) recombinant protein. (Expected MW is 60.2 kDa)

Immunocytochemistry/Immunofluorescence: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis of Hela cells using EIF5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis of rectum cancer tissues using EIF5 mouse mAb with DAB staining.

Western Blot: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis using EIF5 mAb against HEK293 (1) and EIF5 (AA: 1-300)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis of bladder cancer tissues using EIF5 mouse mAb with DAB staining.

ELISA: EIF5 Antibody (6D6H11) [NBP2-61762] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis of Hela cells using EIF5 mouse mAb (green) and negative control (red).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 6D6H11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

EIF5 Antibody (6D6H11) - BSA Free Summary

• Description: Novus Biologicals Mouse EIF5 Antibody (6D6H11) - BSA Free (NBP2-61762) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human EIF5 (AA: 1-300) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 49.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for EIF5 Antibody (6D6H11) - BSA Free

• eIF-5
• EIF-5A
• eukaryotic translation initiation factor 5

Background

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5