eIF5A Antibodies
We offer eIF5A Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each eIF5A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our eIF5A Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the eIF5A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our eIF5A polyclonal antibodies and browse our eIF5A monoclonal antibody catalog.
Alternate Names for eIF5A Antibodies
anti-eIF5A antibody, anti-eIF-4D antibody, anti-EIF-5A antibody, anti-eIF-5A-1 antibody, anti-eIF-5A1 antibody, anti-EIF5A1 antibody, anti-eIF5AI antibody, anti-eukaryotic initiation factor 5A antibody, anti-Eukaryotic initiation factor 5A isoform 1 antibody, anti-eukaryotic translation initiation factor 5A antibody, anti-eukaryotic translation initiation factor 5A-1 antibody, anti-MGC104255 antibody, anti-MGC99547 antibody, anti-Rev-binding factor antibody
10 Results for "eif5a" in Primary Antibodies
![Western Blot: eIF5A Antibody [NBP1-51982] - Analysis of eIF5A in MOLT4 lysate (35 ug protein in RIPA buffer) using NBP1-51982 at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Approx. 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa band according to NP_001137232.1).](http://images.novusbio.com/fullsize/eIF5A-Antibody-Western-Blot-NBP1-51982-img0002.jpg "Western Blot: eIF5A Antibody [NBP1-51982] - Analysis of eIF5A in MOLT4 lysate (35 ug protein in RIPA buffer) using NBP1-51982 at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Approx. 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa band according to NP_001137232.1).")
![Immunohistochemistry-Paraffin: eIF5A Antibody [NBP1-51982] - Staining of paraffin embedded Human Spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/eIF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-51982-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF5A Antibody [NBP1-51982] - Staining of paraffin embedded Human Vessel. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/eIF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-51982-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF5A Antibody [NBP1-51982] - Staining of paraffin embedded Human Tonsil. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/eIF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-51982-img0004.jpg)
![Immunohistochemistry: eIF5A Antibody (5E1) [NBP3-26604] - Image of eIF5A Antibody (5E1) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26604_rabbit-eif5a-mab-5e1-2722024831372.jpg "Immunohistochemistry: eIF5A Antibody (5E1) [NBP3-26604] - Image of eIF5A Antibody (5E1) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: eIF5A Antibody (5E1) [NBP3-26604] - Positive Western Blot detected in: A2780 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate. All lanes: eIF5A Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 17, 21 kDa Observed band size: 18 kDa](http://images.novusbio.com/fullsize/nbp3-26604_rabbit-eif5a-mab-5e1-28220241148586.jpg)
![Immunocytochemistry/Immunofluorescence: eIF5A Antibody (5E1) [NBP3-26604] - Staining of Hela Cells with eIF5A Antibody (5E1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26604_rabbit-eif5a-mab-5e1-28220241247518.jpg)
![Flow Cytometry: eIF5A Antibody (5E1) [NBP3-26604] - Overlay histogram showing Jurkat cells stained with eIF5A Antibody (5E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26604_rabbit-eif5a-mab-5e1-282202414183642.jpg)
![Western Blot: eIF5A Antibody (4D1) [NBP2-59403] - Lane 1: Jurkat cell lysates, Lane 2: 293T cell lysates, Lane 3: HeLa cell lysates, Lane 4: MCF7 cell lysates, Lane 5: NIH-3T3 cell lysates](http://images.novusbio.com/fullsize/eIF5A-Antibody-4D1-Western-Blot-NBP2-59403-img0001.jpg "Western Blot: eIF5A Antibody (4D1) [NBP2-59403] - Lane 1: Jurkat cell lysates, Lane 2: 293T cell lysates, Lane 3: HeLa cell lysates, Lane 4: MCF7 cell lysates, Lane 5: NIH-3T3 cell lysates")
![Immunocytochemistry/Immunofluorescence: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/eIF5A-Antibody-4D1-Immunocytochemistry-Immunofluorescence-NBP2-59403-img0002.jpg)
![Flow Cytometry: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line)](http://images.novusbio.com/fullsize/eIF5A-Antibody-4D1-Flow-Cytometry-NBP2-59403-img0003.jpg)
![Western Blot: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody (M01), clone 8C1.Lane 1: EIF5A transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/eIF5A-Antibody-8C1-Western-Blot-H00001984-M01-img0004.jpg "Western Blot: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody (M01), clone 8C1.Lane 1: EIF5A transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of monoclonal antibody to EIF5A on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/eIF5A-Antibody-8C1-Immunocytochemistry-Immunofluorescence-H00001984-M01-img0002.jpg)
![Sandwich ELISA: eIF5A Antibody (8C1) [H00001984-M01] - Detection limit for recombinant GST tagged EIF5A is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/eIF5A-Antibody-8C1-Sandwich-ELISA-H00001984-M01-img0003.jpg)
![Western Blot: eIF5A Antibody (3R10I7) [NBP3-16409] - Western blot analysis of extracts of various cell lines, using eIF5A Rabbit mAb (NBP3-16409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/eIF5A-Antibody-3R10I7-Western-Blot-NBP3-16409-img0001.jpg "Western Blot: eIF5A Antibody (3R10I7) [NBP3-16409] - Western blot analysis of extracts of various cell lines, using eIF5A Rabbit mAb (NBP3-16409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: eIF5A Antibody [NBP2-38911] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/eIF5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38911-img0002.jpg "Immunohistochemistry-Paraffin: eIF5A Antibody [NBP2-38911] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")