eIF4E Antibody (RM452)

Western Blot: eIF4E Antibody (RM452) [NBP3-23456] - Western Blot of the lysates of MCF-7 cells nontreated (-) or treated (+) with fetal bovine serum, using Clone RM452 at a 1:1000 dilution.

• Reactivity: Hu
• Applications: WB
• Clone: RM452
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

eIF4E Antibody (RM452) Summary

• Description: Novus Biologicals Rabbit eIF4E Antibody (RM452) (NBP3-23456) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A phospho-peptide corresponding to human eIF4E [Ser209] (Uniprot#: P06730 )
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF4E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 -1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for eIF4E Antibody (RM452)

• CBP
• eIF4E
• eIF-4E
• EIF4E1
• EIF4EL1
• EIF4EL1MGC111573
• eIF-4F 25 kDa subunit
• EIF4F
• eukaryotic translation initiation factor 4E
• eukaryotic translation initiation factor 4E-like 1
• mRNA cap-binding protein

Background

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4E