EIF3B Antibody - BSA Free

Western Blot: EIF3B Antibody [NBP2-97742] - Anti-EIF3B rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 120 kDa

Immunoprecipitation: EIF3B Antibody [NBP2-97742] - EIF3B was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 ul anti-EIF3B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EIF3B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 120 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EIF3B Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human EIF3B fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3B
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for EIF3B Antibody - BSA Free

• eIF3 p110
• eIF3 p116
• eIF3b
• eIF-3-eta
• EIF3-P110
• EIF3-P116
• EIF3S9EIF3-ETA
• Eukaryotic translation initiation factor 3 subunit 9
• eukaryotic translation initiation factor 3 subunit B
• eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD)
• eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa
• eukaryotic translation initiation factor 3, subunit B
• MGC104664
• MGC131875
• Prt1 homolog
• PRT1hPrt1

Background

Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF3B