EIF3A Antibody

Images

 
Independent Antibodies: Western Blot: EIF3A Antibody [NBP1-18891] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. eIF3A/EIF3S10 was also immunoprecipitated by rabbit antieIF3A/ EIF3S10 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Xp, ZeSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

EIF3A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human translation initiation factor 3 subunit A (eukaryotic translation initiation factor 3, subunit 10) using the numbering given in entry NP_003741.1 (GeneID 8661).
Predicted Species
Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-18891 in the following applications:

Publications
Read Publications using
NBP1-18891 in the following applications:

  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF3A Antibody

  • centrosomin homolog
  • cytoplasmic protein p167
  • eIF3 p167
  • eIF3 p180
  • eIF3 p185
  • EIF3, p180 subunit
  • eIF3a
  • eIF3-p170
  • EIF3S10EIF3
  • eIF-3-theta
  • eIF3-theta
  • Eukaryotic translation initiation factor 3 subunit 10
  • eukaryotic translation initiation factor 3 subunit A
  • eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD)
  • eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD)
  • eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa
  • eukaryotic translation initiation factor 3, subunit 10, 170kD
  • eukaryotic translation initiation factor 3, subunit A
  • KIAA0139P167
  • p180
  • p185
  • TIF32

Background

Eukaryotic initiation factor 3 subunit A (eIF3A) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5-end of cellular mRNAs near the cap structure (m7GpppN). eIF3A is also known as EIF3S10 (eukaryotic initiation factor 3 subunit 7). eIF3A is the largest subunit of the eIF3 complex. eIF3A expression is regulated through the cell cycle. It's expression peaks in S-phase and it is proposed to play a role in regulating the translation of mRNAs involved in the regulation of cell cycle progression and proliferation. Alternative names for eIF3A include eIF-3-theta, eIF-3 p167, eIF-3 p170, eIF-3 p180, eIF3-p185, EIF3S10, and KIAA0139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Xp, Ze
Applications: WB, IP

Publications for EIF3A Antibody (NBP1-18891)(4)

Review for EIF3A Antibody (NBP1-18891) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-18891:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot EIF3A NBP1-18891
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reviewed by:
Anonymous
WB Other 06/17/2016
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Summary

ApplicationWestern Blot
Sample TestedXenopus brain lysate �, HEK293T cell lysate �, Rat brain lysate �
SpeciesOther

Blocking

Blocking DetailsBlocking Solution: 5% milk in TBST(0.05% Tween20) RT, 1h

Primary Anitbody

Dilution Ratio1:500-1:1000 @ 4C 1-2 O/N in TBST(0.05% Tween20)

Secondary Antibody

Secondary DescriptionGoat Anti-Rabbit IgG (H + L)-HRP Conjugate
Secondary Manufacturer Cat##1706515
Secondary Concentration1:2000

Details

Detection Notesfilm based, ECL, Development Time: 15 mins

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for EIF3A Antibody (NBP1-18891) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for EIF3A Antibody (NBP1-18891)

Discover related pathways, diseases and genes to EIF3A Antibody (NBP1-18891). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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PTMs for EIF3A Antibody (NBP1-18891)

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Blogs on EIF3A

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/17/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol EIF3A
Entrez
Uniprot