EIF2B2 Antibody - BSA Free

Immunohistochemistry-Paraffin: EIF2B2 Antibody [NBP2-38572] - Staining in human thyroid gland and liver tissues using anti-EIF2B2 antibody. Corresponding EIF2B2 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: EIF2B2 Antibody [NBP2-38572] - Staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & focal adhesion sites. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: EIF2B2 Antibody [NBP2-38572] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: EIF2B2 Antibody [NBP2-38572] - Staining of human thyroid gland shows high expression.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EIF2B2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EIF2B2 Antibody - BSA Free (NBP2-38572) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-EIF2B2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: RETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRK
• Predicted Species: Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF2B2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  EIF2B2 Protein (NBP2-38572PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EIF2B2 Antibody - BSA Free

• eIF-2B GDP-GTP exchange factor subunit beta
• EIF2B
• EIF2BB
• EIF-2Bbeta
• eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD)
• eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
• S20I15
• S20III15
• translation initiation factor eIF-2B subunit beta

Background

Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon (EIF2B5; MIM 603945) subunits. EIF2B activates its EIF2 (see MIM 603907) substrate by exchanging EIF2-bound GDP for GTP.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EIF2B2 Antibody (NBP2-38572)(1)

Publication using NBP2-38572

• Ceribelli A, Isailovic N, De Santis M et al. Autoantibodies as biomarkers for interstitial lung disease in idiopathic inflammatory myositis and systemic sclerosis: The case of anti-eIF2B antibodies J Transl Autoimmun 2020-08-04 [PMID: 32743530]

Bioinformatics

• Gene Symbol: EIF2B2
• Uniprot:
  • P49770(Human)