GCN2 [p Thr899] Antibody

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Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GCN2 [p Thr899] Antibody Summary

Immunogen
Phosphopeptide containing human GCN2 T899 site
Modification
p Thr899
Specificity
Detects human GCN2 when phosphorylated at T899 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2AK4
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GCN2 [p Thr899] Antibody

  • EIF2AK4
  • eukaryotic translation initiation factor 2 alpha kinase 4
  • eukaryotic translation initiation factor 2-alpha kinase 4
  • GCN2
  • GCN2GCN2 eIF2alpha kinase
  • GCN2-like protein
  • KIAA1338EC 2.7.11.1

Background

GCN2 (General Control Non-derepressible 2-like protein; also eIF2a kinase 4) is a 190-200 kDa member of the GCN2 subfamily, Ser/Thr kinase family, protein kinase superfamily of proteins  It is widely expressed, being found in skeletal muscle cells, fibroblasts, hepatocytes and neurons.  GCN2 is a key regulator of metabolism under nutrient deprivation.  In particular, when essential amino acids (aa) are limiting, tRNAs exist that are unbound to amino acids.  These “uncharged” tRNAs will bind to GCN2, activating the molecule.  Through GCN2-mediated phosphorylation of eIF2a, promotes GCN4 synthesis, with subsequent activation of amino acid synthesizing enzymes, and a downregulation of lipid storage.  Human GCN2 is 1649 aa in length. It possesses one RWD domain (aa 25-137), two protein kinase domains (aa 296-539 and 590-1001) and an extended His-RS-like region (aa 1021-1492).  There are at least three utilized Ser/Thr phosphorylation sites.  Two isoform variants exist.  One shows a deletion of aa 774-801, while another contains a nine aa substitution for aa 608-1649.  Over aa 22-139, human GCN2 shares 91% aa sequence identity with mouse GCN2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK4