EGLN3/PHD3 Overexpression Lysate (Native)


Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Overexpression Lysate (Adult Normal) [NBL1-10156] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for HIF Prolyl more

Product Details

Reactivity HuSpecies Glossary
Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

EGLN3/PHD3 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC210319 and based on accession number NM_022073.
C-terminal DDK tag
Homo sapiens egl nine homolog 3 (C. elegans) (EGLN3), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NBL1-10156 in the following application:

Read Publication using
NBL1-10156 in the following applications:

  • WB
    1 publication

Lysate Details for EGLN3/PHD3

Protein State

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26801054).


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EGLN3/PHD3 Overexpression Lysate (Native)

  • EC 1.14.11
  • EC 1.14.11.-
  • EGL nine (C.elegans) homolog 3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • egl nine-like protein 3 isoform
  • FLJ21620
  • HIF prolyl hydroxylase 3
  • HIFPH3 MGC125998
  • HIFPH3
  • HIF-PH3
  • HIF-prolyl hydroxylase 3
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 3
  • MGC125999
  • PHD3
  • Prolyl hydroxylase domain-containing protein 3


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for EGLN3/PHD3 Lysate (NBL1-10156)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

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Reviews for EGLN3/PHD3 Lysate (NBL1-10156) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBL1-10156:
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Images Ratings Applications Species Date Details
Western Blot EGLN3/PHD3 NBL1-10156
reviewed by:
WB Human 12/07/2015


ApplicationWestern Blot
Sample TestedHEK293T Whole cell lysate transfected with EGLN3 plasmid


Blocking DetailsPBST 0.2%Tween 20, 5% non fat milk, 30 minutes at Room Temperature

Primary Anitbody

Dilution Ratio1:1000, Overnight, 4 C, PBST with 5%milk

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-HRP
Secondary Manufacturer Cat#SC-2005
Secondary Concentration1:2000


Detection NotesChemiluminescence, G:Box Chemi XLT4, 20 sec, no negative controls


Commentsthis product can be used at much lower concentration than 25 ug.

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Video Protocols

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Contact Information

Product PDFs


Gene Symbol EGLN3

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