EGLN3/PHD3 Overexpression Lysate (Native)

Images

 
Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Overexpression Lysate (Adult Normal) [NBL1-10156] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for HIF Prolyl ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EGLN3/PHD3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210319 and based on accession number NM_022073.
Epitope
C-terminal DDK tag
Specificity
Homo sapiens egl nine homolog 3 (C. elegans) (EGLN3), mRNA.
Gene
EGLN3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-10156 in the following application:

Publications
Read Publication using
NBL1-10156 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26801054).

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EGLN3/PHD3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EGLN3/PHD3 Overexpression Lysate (Native)

  • EC 1.14.11
  • EC 1.14.11.-
  • EGL nine (C.elegans) homolog 3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • egl nine-like protein 3 isoform
  • EGLN3
  • FLJ21620
  • HIF prolyl hydroxylase 3
  • HIFPH3 MGC125998
  • HIFPH3
  • HIF-PH3
  • HIF-prolyl hydroxylase 3
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 3
  • MGC125999
  • pdh3
  • PHD3
  • Prolyl hydroxylase domain-containing protein 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for EGLN3/PHD3 Lysate (NBL1-10156)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for EGLN3/PHD3 Lysate (NBL1-10156) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBL1-10156:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot EGLN3/PHD3 NBL1-10156
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reviewed by:
Anonymous
WB Human 12/07/2015
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Summary

ApplicationWestern Blot
Sample TestedHEK293T Whole cell lysate transfected with EGLN3 plasmid
SpeciesHuman

Blocking

Blocking DetailsPBST 0.2%Tween 20, 5% non fat milk, 30 minutes at Room Temperature

Primary Anitbody

Dilution Ratio1:1000, Overnight, 4 C, PBST with 5%milk

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-HRP
Secondary Manufacturer Cat#SC-2005
Secondary Concentration1:2000

Details

Detection NotesChemiluminescence, G:Box Chemi XLT4, 20 sec, no negative controls

Comments

Commentsthis product can be used at much lower concentration than 25 ug.

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Video Protocols

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FAQs for EGLN3/PHD3 Lysate (NBL1-10156) (0)

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Recent Reviews

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1
4
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3
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2
0
1
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Anonymous
12/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol EGLN3
Entrez