EGLN3/PHD3 Antibody (EG188e/d5) [Biotin]

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Product Details

Summary
Reactivity Hu, Rt, Pm, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-P
Clone
EG188e/d5
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

EGLN3/PHD3 Antibody (EG188e/d5) [Biotin] Summary

Immunogen
Partial recombinant human PHD3/HIF Prolyl Hydroxylase 3 (UniProt# Q9H6Z9)
Localization
Cytoplasm. Nucleus.
Predicted Species
Mouse (98%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EGLN3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This HIF Prolyl Hydroxylase 3 antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen ~27 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Human, monkey and rat. Immunogen sequence has 98% identity to bovine and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EGLN3/PHD3 Antibody (EG188e/d5) [Biotin]

  • EC 1.14.11
  • EC 1.14.11.-
  • EGL nine (C.elegans) homolog 3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • egl nine-like protein 3 isoform
  • EGLN3
  • FLJ21620
  • HIF prolyl hydroxylase 3
  • HIFPH3 MGC125998
  • HIFPH3
  • HIF-PH3
  • HIF-prolyl hydroxylase 3
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 3
  • MGC125999
  • pdh3
  • PHD3
  • Prolyl hydroxylase domain-containing protein 3

Background

HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HIF prolyl hydroxylase 1, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(564) and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may also play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissues, promoting cell death through a caspase-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP1-30440V
DyLight 488 Labeled NBP1-30440G
DyLight 550 Labeled NBP1-30440R
DyLight 650 Labeled NBP1-30440C
FITC Labeled NBP1-30440F
HRP Labeled NBP1-30440H

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Bioinformatics

Gene Symbol EGLN3
Entrez