EGFR Antibody (DH8.3) - EGFRvIII, Mutant

Images

 
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IP, CyTOF-ready
Clone
DH8.3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EGFR Antibody (DH8.3) - EGFRvIII, Mutant Summary

Description
Anti-EGFRvIII antibody clone (DH8.3) recognizes EGFRvIII, a mutated form of EGFR with a truncated extracellular domain found on human tumors. Stabley trans
Immunogen
Synthetic peptide corresponding to the junctional region of the truncated receptor LEEKKGNYVVTDHC,conjugated to keyhole limpet haemcyanin.
Specificity
Clone DH8.3 of EGF R/ErbB1 antibody is specific for Truncated EGFR which is also called EGFR type III, EGFRvIII or delta-EGFR (characterised by a deletion of 267 amino acids in the extracellular domain, leading to a receptor which is unable to bind ligand yet is constitutively active). Because of its impaired internalisation and degradation, EGFRvIII is known to enhance tumourigenesis. NOTE: Clone DH8.3 DOES NOT cross-react with full length or wildtype EGFR (wtEGFR).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EGFR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Publications
Read Publications using
NBP2-50599 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for EGFR Antibody (DH8.3) - EGFRvIII, Mutant

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC 2.7.10.1
  • EGF R
  • EGFR variant III
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1

Background

Epidermal growth factor receptor (EGFR) has attracted considerable attention as a target for cancer therapy. Wild-type (wt)EGFR is amplified in a number of tumor types, and several mutant forms of the coding gene have been found, with delta-EGFR, a deletion mutation lacking exons 2-7 of the external domain, being the most common and particularly associated with glioblastoma. EGF R/ErbB1 antibody (clone DH8.3) recognises the junctional region of the truncated receptor (RGFR typeIII/EGFRvIII/deltaEGFR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGFR Antibody (NBP2-50599)(4)

Reviews for EGFR Antibody (NBP2-50599) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EGFR Antibody (NBP2-50599) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50599AF350
Alexa Fluor 405 NBP2-50599AF405
Alexa Fluor 488 NBP2-50599AF488
Alexa Fluor 532 NBP2-50599AF532
Alexa Fluor 594 NBP2-50599AF594
Alexa Fluor 647 NBP2-50599AF647
Alexa Fluor 700 NBP2-50599AF700
Alexa Fluor 750 NBP2-50599AF750
Allophycocyanin NBP2-50599APC
Allophycocyanin/Cy7 NBP2-50599APCCY7
Biotin NBP2-50599B
DyLight 350 NBP2-50599UV
DyLight 405 NBP2-50599V
DyLight 488 NBP2-50599G
DyLight 550 NBP2-50599R
DyLight 594 NBP2-50599DL594
DyLight 650 NBP2-50599C
DyLight 680 NBP2-50599FR
DyLight 755 NBP2-50599IR
FITC NBP2-50599F
HRP NBP2-50599H
Janelia Fluor 549 NBP2-50599JF549
Janelia Fluor 646 NBP2-50599JF646
PE NBP2-50599PE
PE/Atto594 NBP2-50599PEATT594
PE/Cy5.5 NBP2-50599PECY55
PE/Cy7 NBP2-50599PECY7
PerCP NBP2-50599PCP

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Bioinformatics

Gene Symbol EGFR
Uniprot