Recombinant Rat EGF Protein

SDS-Page: Rat EGF Protein [NBP2-35293]

• Reactivity: Rt
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat EGF Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to EGF, including 3 intramolecular disulfide-bonds Source: E. coli

  Uniprot ID: P07522

  Amino Acid Sequence: NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR

• Details of Functionality: EGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EGF
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of EGF as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 6.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat EGF Protein

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal growth factor (EGF) is the founding member of the EGF family of growth factors. Mature EGF is generated by proteolytic release of a juxtamembrane domain within the transmembrane EGF precursor. It is present in a wide variety of body fluids. EGF binds the receptor ErbB1 and induces signaling through ErbB1 homodimers or heterodimers with ErbB2. It plays a key role in epithelial morphogenesis, angiogenesis, and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for EGF Recombinant Protein (NBP2-35293)(3)

Publications using NBP2-35293

• Qingjie Wang, Leijun Huo, Jinlong He, Wenshuang Ding. Soluble epoxide hydrolase is involved in the development of atherosclerosis and arterial neointima formation by regulating smooth muscle cell migration. Am J Physiol Heart Circ Physiol 2015-12-01 [PMID: 26453326]
• Feifei Liu, Aiguo Xuan, Yan Chen, Jundu Zhang. Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms. Mol Med Rep 2014-10-01 [PMID: 25051506]
• Wang Y, Shen X, Liao W et al. A heteropolysaccharide, L-fuco-D-manno-1,6-a-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative. Carbohydr Polym 2014-01-30 [PMID: 24299820]

Bioinformatics

• Gene Symbol: EGF
• Uniprot:
  • P07522()
  • P07522()
  • P07522()