EGF Antibodies

236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND₅₀ is typically 0.05-0.1 µg/mL." class="big_lightbox"> 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND₅₀ is typically 0.05-0.1 µg/mL." class="big_thumb" />

2028-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse EGF (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028). The ND₅₀ is typically 0.02-0.06 µg/mL." class="big_lightbox"> 2028-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse EGF (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028). The ND₅₀ is typically 0.02-0.06 µg/mL." alt="Recombinant Mouse EGF (Catalog # 2028-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse EGF (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028). The ND₅₀ is typically 0.02-0.06 µg/mL." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

2028-EG) was serially diluted and captured by Rat Anti-Mouse EGF Monoclonal Antibody (Catalog # MAB2028) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2028-EG) was serially diluted and captured by Rat Anti-Mouse EGF Monoclonal Antibody (Catalog # MAB2028) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236). The ND₅₀ is typically 0.040 - 0.480 µg/mL." class="big_lightbox"> 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236). The ND₅₀ is typically 0.040 - 0.480 µg/mL." class="big_thumb" />

236-EG) was serially diluted and captured by Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 236-EG) was serially diluted and captured by Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

236-EG) was serially diluted and captured by Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 236-EG) was serially diluted and captured by Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> Fluorescent IHC Staining of Frozen Tissue Sections." alt="Pro Epidermal Growth Factor (Pro EGF) was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Pro EGF Monoclonal Antibody (Catalog # MAB5256) at 10 µg/mL for 3 hours at room temperature. Tissue was stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

3214-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat EGF (75 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat EGF Monoclonal Antibody (Catalog # MAB3214). The ND₅₀ is typically 0.75-3.75 µg/mL." class="big_lightbox"> 3214-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat EGF (75 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat EGF Monoclonal Antibody (Catalog # MAB3214). The ND₅₀ is typically 0.75-3.75 µg/mL." alt="Recombinant Rat EGF (Catalog # 3214-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat EGF (75 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat EGF Monoclonal Antibody (Catalog # MAB3214). The ND₅₀ is typically 0.75-3.75 µg/mL." class="big_thumb" />

3214-EG) was serially diluted and captured by Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 3214-EG) was serially diluted and captured by Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Rat EGF (Catalog # 3214-EG) was serially diluted and captured by Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

3214-EG) was serially diluted and captured by Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 3214-EG) was serially diluted and captured by Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Rat EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3214) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2028-EG) was serially diluted and captured by Rat Anti-Mouse EGF Monoclonal Antibody (Catalog # MAB2028) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2028-EG) was serially diluted and captured by Rat Anti-Mouse EGF Monoclonal Antibody (Catalog # MAB2028) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse EGF (Catalog # 2028-EG) was serially diluted and captured by Rat Anti-Mouse EGF Monoclonal Antibody (Catalog # MAB2028) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />