Recombinant Human EGF Protein

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SDS-Page: Human EGF Protein [NBP2-34952]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Human EGF Protein Summary

Description
A single, non-glycosylated polypeptide chain corresponding to 53 residues of EGF.

Source: Escherichia coli

Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
EGF
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of EGF as determined by LAL method.

Applications/Dilutions

Theoretical MW
6.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34952 in the following application:

Publications
Read Publications using NBP2-34952.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human EGF Protein

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

EGF R (ErbB1) is a receptor tyrosine kinase that is widely expressed on epithelial cells and is required for epithelial cell development and proliferation. EGF R binds the EGF family ligands: EGF, Amphiregulin, TGF-alpha, Betacellulin, Epiregulin, HB-EGF, and Epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, ErbB3, or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Alterations in the structure, expression, and signaling of EGF R are involved in the development and metastasis of a wide variety of cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for EGF Recombinant Protein (NBP2-34952)(12)


Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP2-34952 Applications Species
Robilliard LD, Yu J, Anchan A et al. Comprehensive analysis of inhibitory checkpoint ligand expression by Glioblastoma cells Immunol Cell Biol Nov 20 2020 [PMID: 33217047]
Wucheng Wang, Huanjun Chen, Lei Zhang et al. A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4. Carbohydr Polym 2016 Jun 25 [PMID: 27083822]
Liu, Wanjun PhD, Wang et al. CYP2J2 Overexpression Increases EETs and Protects Against HFD-Induced Atherosclerosis in ApoE−/− Mice. Journal of Cardiovascular Pharmacology 2016 Jun [PMID: 26859195]
Yan Luan, Dafei Chai, Jianjian Peng et al. A fully human monoclonal antibody targeting PD-L1 with potent anti-tumor activity. Int Immunopharmacol 2016 Feb [PMID: 26773772]
Jiayin Hou, Liang Liu, Qian Zhu et al. MicroRNA‐185 inhibits angiogenesis in human microvascular endothelial cells through targeting stromal interaction molecule 1. Cell Biol Int 2016 Mar [PMID: 26694763]
Yu Ma, Fu-Chun Guo, Wei Wang et al. K-ras gene mutation as a predictor of cancer cell responsiveness to metformin. Mol Med Rep 2013 Sep [PMID: 23877793]
Xia Chen, Fei Xiao, Ying Wang et al. Structure-activity relationship study of WSS25 derivatives with anti-angiogenesis effects. Glycoconj J 2012 Aug [PMID: 22847113]
Yuxia Xu, Qun Dong, Hong Qiu et al. A homogalacturonan from the radix of Platycodon grandiflorum and the anti-angiogenesis activity of poly-/oligogalacturonic acids derived therefrom. Carbohydr Res 2011 Sep 27 [PMID: 21700274]
Xiaokun Shen, Jianping Fang, Xiaofen Lv et al. Heparin Impairs Angiogenesis through Inhibition of MicroRNA-10b. J Biol Chem 2011 Jul 29 [PMID: 21642433]
Yuxia Xu, Qun Dong, Hong Qiu et al. Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative. Biomacromolecules 2010 Oct 11 [PMID: 20839877]
Show All 12 Publications.

Review for EGF Recombinant Protein (NBP2-34952) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-34952:
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5
reviewed by:
Verified Customer
01/04/2017
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Summary

CommentsI ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

CommentsSDS PAGE gel with Coomassie blue staining showing detection of Human EGF protein (NBP2-34952-100 ug ) (5 ug).

FAQs for EGF Recombinant Protein (NBP2-34952) (0)

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Gene Symbol EGF
Uniprot