EBI3 Antibody - BSA Free

Western Blot: EBI3 Antibody [NBP1-76976] - Analysis of EBI3 in human tonsil tissue lysate with EBI3 antibody at 2 ug/mL.

Western Blot: EBI3 Antibody [NBP1-76976] - K562 lysate with EBI3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Western Blot: EBI3 Antibody - BSA Free [NBP1-76976] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane.Antibodies: EBI-3 , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 ug/mL, Lane 2: 2 ug/mL, Lane 3: 4 ug/mL,

Immunohistochemistry: EBI3 Antibody - BSA Free [NBP1-76976] - Validation of EBI-3 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded Human Spleen tissue using anti-EBI-3 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: EBI3 Antibody - BSA Free [NBP1-76976] - Validation of EBI-3 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen tissue labeling EBI-3 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

EBI3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EBI3 Antibody - BSA Free (NBP1-76976) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-EBI3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The immunogen is located within the last 50 amino acids of EBI3. Amino Acid Squence: ILRAVRPRATYYIQV
• Specificity: Human EBI3 has only 1 isoform (229aa, 25kD). Mouse EBI3 has one isoform (228aa, 25kD) and rat EBI3 also has one isoform (228aa, 25kD). NBP1-76976 can detect human EBI3 but cannot detect mouse and rat EBI3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EBI3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-4 ug/ml
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  EBI3 Protein (NBP1-76976PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for EBI3 Antibody - BSA Free

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBI3 Antibody (NBP1-76976)(1)

Publication using NBP1-76976

• Carbotti G, Barisione G, Orengo AM et al. The IL-18 Antagonist IL-18-Binding Protein Is Produced in the Human Ovarian Cancer Microenvironment. Clin Cancer Res. 2013-09-01 [PMID: 23873689] (IHC-P, Mouse, Human)

Bioinformatics

• Gene Symbol: EBI3
• Uniprot:
  • NP_005746()