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39 Results for "il 27 il 35 ebi3 subunit"
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MAB18341

EBI3 Antibody (355022)

(8 Publications)
100 μg
MAB18341
Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with mouse EBI3. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit Monoclonal Antibody (Catalog # MAB18341) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-27/IL-35 EBI3 Subunit at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB18341U-100UG
Alexa Fluor 405 FAB18341V-100UG
Alexa Fluor 488 FAB18341G-100UG
Alexa Fluor 532 FAB18341X-100UG
Alexa Fluor 594 FAB18341T-100UG
Alexa Fluor 647 FAB18341R-100UG
Alexa Fluor 700 FAB18341N-100UG
Alexa Fluor 750 FAB18341S-100UG
Allophycocyanin IC18341A
Biotin FAB18341B
CoraFluor 1 FAB18341CL1
DyLight 350 FAB18341D
DyLight 405 FAB18341E
DyLight 488 FAB18341K
DyLight 550 FAB18341L
DyLight 594 FAB18341M
DyLight 650 FAB18341W
DyLight 680 FAB18341Y
DyLight 755 FAB18341Z
FITC FAB18341F
HRP FAB18341H
Janelia Fluor 525 FAB18341JF525
Janelia Fluor 549 FAB18341I
Janelia Fluor 646 FAB18341J
Janelia Fluor 669 FAB18341JF669
PerCP (Peridinin-chlorophyll Protein Complex) IC18341C
Phycoerythrin IC18341P
mFluor Violet 450 SE FAB18341MFV450
mFluor Violet 500 SE FAB18341MFV500
mFluor Violet 610 SE FAB18341MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC18341C
Carrier Free IC18341P
Carrier Free IC18341A
Carrier Free MAB18341-SP
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MAB6456

EBI3 Antibody (607201)

(2 Publications)
100 μg
MAB6456
KG‑1 human acute myelogenous leukemia cell line treated with 40 ng/mL Recombinant Human IL‑18/IL‑1F4 (Catalog # B001-5) for 24 hrs was stained with Human IL‑27/IL-35 EBI3 Subunit Monoclonal Antibody (Catalog # MAB6456, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Hu
Species Glossary
Reactivity: Hu
CyTOF-ready, Flow
Applications: CyTOF-ready, Flow
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC6456U-100UG
Alexa Fluor 405 IC6456V-100UG
Alexa Fluor 488 IC6456G
Alexa Fluor 532 FAB6456X
Alexa Fluor 594 IC6456T-100UG
Alexa Fluor 647 IC6456R-100UG
Alexa Fluor 700 IC6456N-100UG
Alexa Fluor 750 IC6456S-100UG
Allophycocyanin IC6456A
Biotin FAB6456B
CoraFluor 1 FAB6456CL1
DyLight 350 FAB6456D
DyLight 405 FAB6456E
DyLight 488 FAB6456K
DyLight 550 FAB6456L
DyLight 594 FAB6456M
DyLight 650 FAB6456W
DyLight 680 FAB6456Y
DyLight 755 FAB6456Z
FITC FAB6456F
HRP FAB6456H
Janelia Fluor 525 FAB6456JF525
Janelia Fluor 549 FAB6456I
Janelia Fluor 646 FAB6456J
Janelia Fluor 669 FAB6456JF669
PE FAB6456P
PerCP FAB6456C
mFluor Violet 450 SE FAB6456MFV450
mFluor Violet 500 SE FAB6456MFV500
mFluor Violet 610 SE FAB6456MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC6456A
Carrier Free IC6456G
Carrier Free MAB6456-SP
Carrier Free IC6456N-100UG
Carrier Free IC6456V-100UG
Carrier Free IC6456U-100UG
Carrier Free IC6456T-100UG
Carrier Free IC6456S-100UG
Carrier Free IC6456R-100UG
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11084-EI

Recombinant Human EBI3 Protein, CF

50 μg
11084-EI
Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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MAB64561

EBI3 Antibody (607215) [Unconjugated]

100 μg
MAB64561
Recombinant Human IL‑39 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL‑23 p19 Monoclonal Antibody (MAB17161) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human EBI3 Monoclonal Antibody (Catalog # MAB64561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB64561U-100UG
Alexa Fluor 405 FAB64561V-100UG
Alexa Fluor 488 FAB64561G-100UG
Alexa Fluor 532 FAB64561X-100UG
Alexa Fluor 594 FAB64561T-100UG
Alexa Fluor 647 FAB64561R-100UG
Alexa Fluor 700 FAB64561N-100UG
Alexa Fluor 750 FAB64561S-100UG
Allophycocyanin FAB64561A
Biotin FAB64561B
CoraFluor 1 FAB64561CL1
DyLight 350 FAB64561D
DyLight 405 FAB64561E
DyLight 488 FAB64561K
DyLight 550 FAB64561L
DyLight 594 FAB64561M
DyLight 650 FAB64561W
DyLight 680 FAB64561Y
DyLight 755 FAB64561Z
FITC FAB64561F
HRP FAB64561H
Janelia Fluor 525 FAB64561JF525
Janelia Fluor 549 FAB64561I
Janelia Fluor 646 FAB64561J
Janelia Fluor 669 FAB64561JF669
PE FAB64561P
PerCP FAB64561C
mFluor Violet 450 SE FAB64561MFV450
mFluor Violet 500 SE FAB64561MFV500
mFluor Violet 610 SE FAB64561MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB64561-SP
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DY6456-05

Human EBI3 DuoSet ELISA, 5 Plate

(1 Publication)
1 Kit (for 5 Plates)
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
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IC18341A

EBI3 Antibody (355022) [Allophycocyanin]

(2 Publications)
100 Tests
IC18341A
Mouse splenocytes untreated (open histogram) or treated with LPS (filled histogram) were stained with Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Monoclonal Antibody (Catalog # IC18341A) or isotype control antibody (Catalog # IC006A, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Mu
Species Glossary
Reactivity: Mu
Flow
Applications: Flow
Rat Monoclonal
Host: Rat Monoclonal
Allophycocyanin  
Available Conjugates:
Conjugate Catalog # Availability Size Price
PerCP (Peridinin-chlorophyll Protein Complex) IC18341C
Phycoerythrin IC18341P
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC18341C
Carrier Free IC18341P
Carrier Free MAB18341
Carrier Free MAB18341-SP
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FAB18341V

EBI3 Antibody (355022) [Alexa Fluor® 405]

100 μg
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Rat Monoclonal
Host: Rat Monoclonal
Alexa Fluor 405  
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FAB18341T

EBI3 Antibody (355022) [Alexa Fluor® 594]

100 μg
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Rat Monoclonal
Host: Rat Monoclonal
Alexa Fluor 594  
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FAB18341N

EBI3 Antibody (355022) [Alexa Fluor® 700]

100 μg
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Rat Monoclonal
Host: Rat Monoclonal
Alexa Fluor 700  
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FAB18341X

EBI3 Antibody (355022) [Alexa Fluor® 532]

100 μg
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Rat Monoclonal
Host: Rat Monoclonal
Alexa Fluor 532  
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FAB18341S

EBI3 Antibody (355022) [Alexa Fluor® 750]

100 μg
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Rat Monoclonal
Host: Rat Monoclonal
Alexa Fluor 750  
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FAB18341R

EBI3 Antibody (355022) [Alexa Fluor® 647]

100 μg
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Rat Monoclonal
Host: Rat Monoclonal
Alexa Fluor 647  
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