E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5))

E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, IP, B/N, CyTOF-ready, ICC/IF
• Clone: BBIG-E6 (13D5)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5)) Summary

• Immunogen: Activated HUVEC human umbilical vein endothelial cells
• Specificity: Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5))

• CD62E
• ELAM
• ELAM1
• ESEL
• Eselectin
• E-Selectin/P-Selectin
• LECAM2
• PSelectin

Background

E-Selectin and P-Selectin are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBA1)(20)

Publications using BBA1

• Koshrovski-Michael, S;Ajamil, DR;Dey, P;Kleiner, R;Tevet, S;Epshtein, Y;Green Buzhor, M;Khoury, R;Pozzi, S;Shenbach-Koltin, G;Yeini, E;Woythe, L;Blau, R;Scomparin, A;Barshack, I;Florindo, HF;Lazar, S;Albertazzi, L;Amir, RJ;Satchi-Fainaro, R; Two-in-one nanoparticle platform induces a strong therapeutic effect of targeted therapies in P-selectin-expressing cancers Science advances 2024-12-13 [PMID: 39671487] (Immunohistochemistry, Human)
• Kintos, DP;Salagiannis, K;Sgouros, A;Nikolaropoulos, SS;Topouzis, S;Fousteris, MA; Identification of new multi-substituted 1H-pyrazolo[3,4-c]pyridin-7(6H)-ones as soluble guanylyl cyclase (sGC) stimulators with vasoprotective and anti-inflammatory activities Bioorganic chemistry 2024-02-02 [PMID: 38335755] (ELISA Capture, Rat)
• Orianne Constant, Ghizlane Maarifi, Jonathan Barthelemy, Marie-France Martin, Bachirou Tinto, Giovanni Savini et al. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity Emerging Microbes & Infections 2023-12-31 [PMID: 36495563] (Flow Cytometry, Insect - Aedes albopictus (Asian tiger mosquito))
• Orianne Constant, Ghizlane Maarifi, Jonathan Barthelemy, Marie-France Martin, Bachirou Tinto, Giovanni Savini et al. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity Emerging Microbes & Infections 12/31/2023 [PMID: 36495563]
• A Krivitsky, S Pozzi, E Yeini, S Israeli Da, T Zur, S Golan, V Krivitsky, N Albeck, E Pisarevsky, P Ofek, A Madi, R Satchi-Fai Sulfonated Amphiphilic Poly(alpha)glutamate Amine-A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors Pharmaceutics, 2021-12-20;13(12):. 2021-12-20 [PMID: 34959480] (Flow Cytometry, IHC, Human)
• L Neufeld, E Yeini, N Reisman, Y Shtilerman, D Ben-Shusha, S Pozzi, A Madi, G Tiram, A Eldar-Booc, S Ferber, R Grossman, Z Ram, R Satchi-Fai Microengineered perfusable 3D-bioprinted glioblastoma model for in vivo mimicry of tumor microenvironment Science Advances, 2021-08-18;7(34):. 2021-08-18 [PMID: 34407932] (Flow Cytometry, IHC, Human)
• E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression Nature Communications, 2021-03-26;12(1):1912. 2021-03-26 [PMID: 33771989] (ICC, Human)
• M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier MBio, 2020-08-04;11(4):. 2020-08-04 [PMID: 32753493] (Neutralization, Human)
• RA Sabol, VA Villela, A Denys, BT Freeman, AB Hartono, RM Wise, MAA Harrison, MB Sandler, F Hossain, L Miele, BA Bunnell Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling Int J Mol Sci, 2020-04-15;21(8):. 2020-04-15 [PMID: 32326381]
• JD Belcher, C Chen, J Nguyen, F Abdulla, P Zhang, H Nguyen, P Nguyen, T Killeen, SM Miescher, N Brinkman, KA Nath, CJ Steer, GM Vercellott Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction PLoS ONE, 2018-04-25;13(4):e0196455. 2018-04-25 [PMID: 29694434] (ICC, Human)
• HC Morrogh-Be, I Foitová, Z Yeen, P Wilkin, R de Martin, L Rárová, K Doležal, W Nurcahyo, M Olšanský Self-medication by orang-utans (Pongo pygmaeus) using bioactive properties of Dracaena cantleyi Sci Rep, 2017-11-30;7(1):16653. 2017-11-30 [PMID: 29192145] (ICC, Human)
• Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells PLoS ONE, 2016-12-01;11(12):e0167361. 2016-12-01 [PMID: 27907087] (Flow Cytometry, Human)
• SDF-1?-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia Sci Rep, 2016-10-07;6(0):34416. 2016-10-07 [PMID: 27713493] (Neutralization, Human)
• Zhao-Jun Liu, Pirouz Daftarian, Letícia Kovalski, Bo Wang, Runxia Tian, Diego M. Castilla et al. Directing and Potentiating Stem Cell-Mediated Angiogenesis and Tissue Repair by Cell Surface E-Selectin Coating PLOS ONE 4/22/2016 [PMID: 27104647]
• Joanna L. MacKay, Daniel A. Hammer Stiff substrates enhance monocytic cell capture through E-selectin but not P-selectin Integrative Biology 1/1/2016 [PMID: 26626697]
• Jorgensen MM, Baek R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 1/1/2015 [PMID: 25862471]
• Roe , Kelsey, Orillo , Beverly, Verma , Saguna West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model. PLoS ONE, 2014-07-18;9(7):e102598. 2014-07-18 [PMID: 25036379] (Neutralization, Human)
• Duluc , D, Gannevat , J, Anguiano , E, Zurawski , S, Carley , M, Boreham , M, Stecher , J, Dullaers , M, Banchereau , J, Oh , S Functional diversity of human vaginal APC subsets in directing T-cell responses. Mucosal Immunol, 2012-11-07;6(3):626-38. 2012-11-07 [PMID: 23131784] (Flow Cytometry, Human)
• Eric K. Sackmann, Erwin Berthier, Edmond W.K. Young, Miriam A. Shelef, Sarah A. Wernimont, Anna Huttenlocher et al. Microfluidic kit-on-a-lid: a versatile platform for neutrophil chemotaxis assays Blood 10/4/2012 [PMID: 22915642]
• Cunningham DA, Dos Santos Cruz GJ, Fernandez-Suarez XM, Whittam AJ, Herring C, Copeman L, Richards A, Langford GA Activation of primary porcine endothelial cells induces release of porcine endogenous retroviruses. Transplantation, 2004-04-15;77(7):1071-9. 2004-04-15 [PMID: 15087774] (Flow Cytometry, Porcine)