E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5)) [Phycoerythrin]

HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF-a (Catalog # 210-TA) were stained with Mouse Anti-Human E-Selectin/P-Selectin (CD62E/P) PE-conjugated Monoclonal Antibody (Catalog # FAB6169P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: BBIG-E6 (13D5)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5)) [Phycoerythrin] Summary

• Immunogen: Activated HUVEC human umbilical vein endothelial cells
• Specificity: Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5)) [Phycoerythrin]

• CD62E
• ELAM
• ELAM1
• ESEL
• Eselectin
• E-Selectin/P-Selectin
• LECAM2
• PSelectin

Background

E-Selectin (CD62E) and P-Selectin (CD62P) are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (FAB6169P)(2)

Publications using FAB6169P

• Jia, H;Moore, M;Wadhwa, M;Burns, C; Human iPSC-Derived Endothelial Cells Exhibit Reduced Immunogenicity in Comparison With Human Primary Endothelial Cells Stem cells international 2024-12-09 [PMID: 39687754] (Flow Cytometry, Human)
• Kevin Wilhelmsen, Kailin R Mesa, Arun Prakash, Fengyun Xu, Judith Hellman Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response Innate Immunity 2012-08-01 [PMID: 22186927]