E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5)) [Fluorescein]

HUVEC human umbilical vein endothelial cells stained with TNF-a was stained with Mouse Anti-Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB6169F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: BBIG-E6 (13D5)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5)) [Fluorescein] Summary

• Immunogen: Activated HUVEC human umbilical vein endothelial cells
• Specificity: Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (BBIG-E6 (13D5)) [Fluorescein]

• CD62E
• ELAM
• ELAM1
• ESEL
• Eselectin
• E-Selectin/P-Selectin
• LECAM2
• PSelectin

Background

E-Selectin (CD62E) and P-Selectin (CD62P) are members of the Selectin/LECAM family of molecules. E- and P-Selectin are type I transmembrane glycoproteins that contain an N-terminal calcium-dependent C-type lectin domain, one EGF-like repeat, multiple sushi domains, and a transmembrane segment followed by a short cytoplasmic tail. Typically, the ligands for E-and P-Selectin are carbohydrates that contain a sialyl-Lewis X (sLe^X) motif that appears on select O- and N-linked glycans and glycosphingolipids. Human E-Selectin is 95-115 kDa is size and expressed by vascular endothelium. It has a number of ligands, not all of which are shared between the human and rodent systems. For example human E-Selectin binds L-Selectin, CD44/HCELL and a poorly characterized pronase-insensitive ganglioside, none of which are recognized by mouse E-Selectin. By contrast, mouse E-Selectin binds E-Selectin Ligand whereas human E-Selectin does not. Other ligands identified for human E-Selectin include PSGL-1, CD43, Mac-1, CD66, Endoglycan and Podocalyxin-like protein. Human P-Selectin is 130-140 kDa in size, and expressed platelets and vascular endothelium. It primarily binds sLe^X on O-glycans, including PSGL-1 and endoglycan. Functionally, Selectins induce leukocyte rolling on endothelial cell surfaces, an effect that activates neighboring alpha 2 beta 2/LFA-1 and slows leukocyte migration. Although the extracellular domains of human E- and P-Selectin show only 36% amino acid (aa) sequence identity, over the first 180 aa (out of >500 aa), E- and P- Selectin demonstrate 62% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin (CD62E)/P-Selectin (CD62P) Antibody (FAB6169F)(2)

Publications using FAB6169F

• N Farahi, E Paige, J Balla, E Prudence, RC Ferreira, M Southwood, SL Appleby, P Bakke, A Gulsvik, AA Litonjua, D Sparrow, EK Silverman, MH Cho, J Danesh, DS Paul, DF Freitag, ER Chilvers Neutrophil-mediated IL-6 receptor trans-signaling and the risk of chronic obstructive pulmonary disease and asthma Hum. Mol. Genet., 2017-04-15;26(8):1584-1596. 2017-04-15 [PMID: 28334838] (Flow Cytometry, Human)
• Lauridsen H, Pober J, Gonzalez A A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment. FASEB J, 2013-12-02;28(3):1166-80. 2013-12-02 [PMID: 24297702] (ICC, Human)