DREF Antibody (PCRP-ZBED1-1E1)

Immunocytochemistry/Immunofluorescence: DREF Antibody (PCRP-ZBED1-1E1) [NBP3-07536] - Immunofluorescence Analysis of PFA-fixed HeLa cells using DREF Mouse Monoclonal Antibody (PCRP-ZBED1-1E1) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: DREF Antibody (PCRP-ZBED1-1E1) [NBP3-07536] - Flow Cytometric Analysis of PFA-fixed HeLa cells. DREF Mouse Monoclonal Antibody (PCRP-ZBED1-1E1) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Immunocytochemistry/Immunofluorescence: DREF Antibody (PCRP-ZBED1-1E1) [NBP3-07536] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using DREF Mouse Monoclonal Antibody (PCRP-ZBED1-1E1) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Protein Array: DREF Antibody (PCRP-ZBED1-1E1) [NBP3-07536] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DREF Mouse Monoclonal Antibody (PCRP-ZBED1-1E1).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, MA
• Clone: PCRP-ZBED1-1E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DREF Antibody (PCRP-ZBED1-1E1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08861)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human DREF protein (Uniprot: O96006)
• Localization: Nucleus. In granular structures.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZBED1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for DREF Antibody (PCRP-ZBED1-1E1)

• Ac-like transposable element
• ALTEDNA replication-related element binding factor
• hDREF
• KIAA0785dREF homolog
• Putative Ac-like transposable element
• putative Ac-like transposon
• TRAMPDREFBED-type zinc finger domain-containing protein 1
• zinc finger BED domain-containing protein 1
• zinc finger, BED-type containing 1

Background

DREF binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable el

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBED1
• Uniprot:
  • O96006(Human)