SKIV2L2 Antibody

Images

 
Western Blot: SKIV2L2 Antibody [NB100-1574] - Detection of Human and Mouse SKIV2L2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN ...read more
Immunohistochemistry: SKIV2L2 Antibody [NB100-1574] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-expndtw-6 SKIV2L2 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: SKIV2L2 Antibody [NB100-1574] - Detection of Human and Mouse SKIV2L2 on HeLa whole cell lysate using NB100-1574. SKIV2L2 was successfully immunoprecipitated using anti-SKIV2L2 antibody NB100-1575 at 3 ...read more
Western Blot: SKIV2L2 Antibody [NB100-1574] - Analysis of SKIV2L2 in HeLa cell lysates treated with (1) si-control and (2) SKIV2L2 siRNA using anti-SKIV2L2 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NB100-1574] - Detection of Human SKIV2L2 by Immunohistochemistry. Sample: FFPE section of human lung adenocarcinoma. Antibody: Affinity purified rabbit anti-SKIV2L2 used ...read more
Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NB100-1574] - Detection of Mouse SKIV2L2 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-SKIV2L2 used at a ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
   

Biological Strategies

 

Genetic Strategies

   

Order Details

SKIV2L2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human SuperKiller Viralicidic Activity 2-Like 2 using the numbering given in entry NP_056175.2 (GeneID 23517).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SKIV2L2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100- 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2 - 10 Ug/mg lysate
Application Notes
For WB: When blotting for mouse SKIV2L2, lower dilutions of antibody and/or longer exposure times may be required.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-1574 in the following applications:

Publications
Read Publications using
NB100-1574 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SKIV2L2 Antibody

  • ATP-dependent helicase SKIV2L2
  • Dob1
  • EC 3.6.1
  • EC 3.6.4.13
  • fSAP118
  • KIAA0052functional spliceosome-associated protein 118
  • MGC142069
  • Mtr4superkiller viralicidic activity 2-like 2
  • superkiller viralicidic activity 2-like 2 (S. cerevisiae)

Background

SKIV2L2 is also known as hMtr4 and KIAA0052 and is part of the nuclear exosome involved in ribosomal RNA processing. Alternate designations for SKIV2L2 include superkiller viralicidic activity 2-like 2, ATP-dependent helicase SKIV2L2, KIAA0052, Dob1, and MGC142069.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SKIV2L2 Antibody (NB100-1574)(3)

Review for SKIV2L2 Antibody (NB100-1574) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-1574:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SKIV2L2 NB100-1574
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reviewed by:
Anonymous
WB Human 05/05/2015
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking DetailsTBS, 5% skim milk, 1h, r.t.

Primary Anitbody

Dilution Ratio1:2000, O/N, 4C, 5% skim milk in TBS

Details

Detection NotesECL, 30sec-1min exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SKIV2L2 Antibody (NB100-1574) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SKIV2L2 Antibody (NB100-1574)

Discover related pathways, diseases and genes to SKIV2L2 Antibody (NB100-1574). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SKIV2L2 Antibody (NB100-1574)

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Blogs on SKIV2L2

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Recent Reviews

5
5
1
4
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3
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2
0
1
0

Anonymous
05/05/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol SKIV2L2
Entrez
Uniprot