SKIV2L2 Antibody

Western Blot: SKIV2L2 Antibody [NB100-1574] - Analysis of SKIV2L2 in HeLa cell lysates treated with (1) si-control and (2) SKIV2L2 siRNA using anti-SKIV2L2 antibody. Image from verified customer review.

Western Blot: SKIV2L2 Antibody [NB100-1574] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiSKIV2L2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NB100-1574] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-SKIV2L2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

SKIV2L2 Antibody [NB100-1574] - Detection of human SKIV2L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SKIV2L2 antibody NB100-1574 used for IP at 3 ug per reaction. SKIV2L2 was also immunoprecipitated by rabbit anti-SKIV2L2 antibody NB100-1575. For blotting immunoprecipitated SKIV2L2, NB100-1574 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

SKIV2L2 Antibody [NB100-1574] - Affinity purified rabbit anti-SKIV2L2 used at a dilution of 1:200 (1ug/ml).

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ICC/IF, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SKIV2L2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human SuperKiller Viralicidic Activity 2-Like 2 using the numbering given in entry NP_056175.2 (GeneID 23517).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTREX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:100 - 1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
• Application Notes: For WB: When blotting for mouse SKIV2L2, lower dilutions of antibody and/or longer exposure times may be required. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. SKIV2L2 antibody validated for WB from a verified customer review.
• Theoretical MW: 118 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SKIV2L2 Antibody

• ATP-dependent helicase SKIV2L2
• Dob1
• EC 3.6.1
• EC 3.6.4.13
• fSAP118
• KIAA0052functional spliceosome-associated protein 118
• MGC142069
• Mtr4superkiller viralicidic activity 2-like 2
• superkiller viralicidic activity 2-like 2 (S. cerevisiae)

Background

SKIV2L2 is also known as hMtr4 and KIAA0052 and is part of the nuclear exosome involved in ribosomal RNA processing. Alternate designations for SKIV2L2 include superkiller viralicidic activity 2-like 2, ATP-dependent helicase SKIV2L2, KIAA0052, Dob1, and MGC142069.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SKIV2L2 Antibody (NB100-1574)(5)

Publications using NB100-1574

• Ogami K, Richard P, Chen Y et al. An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression Genes Dev. 2017 Jul 21 [PMID: 28733371]
• Ogami K, Richard P, Chen Y et al. An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. Gens & Dev. 2017 Jul 21 [PMID: 28733371]
• Ogami K, Richard P, Chen Y et al. An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. Gens & Dev. 2017-07-21 [PMID: 28733371] (Western Blot, Human)
• Nag A, Steitz JA. Tri-snRNP-associated proteins interact with subunits of the TRAMP and nuclear exosome complexes, linking RNA decay and pre-mRNA splicing. RNA Biol. 2012-03-01 [PMID: 22336707] (WB, Human)
• Chen Y Studies on RNA Regulation: From Enhancer RNAs to RBBP6 Isoform3 Thesis 2019-01-01

Review for SKIV2L2 Antibody (NB100-1574) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 05/05/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: MTREX
• Entrez:
  • 23517(Human)
  • 72198(Mouse)
• Uniprot:
  • P42285(Human)