DOT1L Antibody - BSA Free

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Immunocytochemistry/ Immunofluorescence: DOT1L Antibody [NBP2-55244] - Staining of human cell line U-2 OS shows localization to nucleoplasm.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

DOT1L Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit DOT1L Antibody - BSA Free (NBP2-55244) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LLKARCEELQLDWATLSLEKLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDDALSLHLRGKGALGRELEPDASRLHLELDCTKFSLPHLSSMSPELSMNGQAAGYELCGVLSRPSSKQNTPQYLASPLDQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DOT1L
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DOT1L Recombinant Protein Antigen (NBP2-55244PEP)

Reactivity Notes

Mouse 88%, Rat 89%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for DOT1L Antibody - BSA Free

  • DOT1L
  • DOT1-like protein
  • DOT1-like, histone H3 methyltransferase (S. cerevisiae)
  • EC 2.1.1.43
  • H3-K79-HMTase
  • KIAA1814DKFZp586P1823
  • KMT4
  • KMT4H3 lysine-79 specific
  • Lysine N-methyltransferase 4

Background

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DOT1L