RNF20 Antibody

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Biological Strategies: Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human and Mouse RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using ...read more
Western Blot: RNF20 Antibody [NB100-2242] - WB analysis of RNF20 in HeLa whole cell lysate. Image submitted by a verified customer review.
Independent Antibodies: Immunoprecipitation: RNF20 Antibody [NB100-2242] - Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RNF20 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF20
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Chip reactivity reported in (PMID: 21617703).
Reviewed Applications
Read 1 Review rated 4
using
NB100-2242 in the following applications:

Publications
Read Publications using
NB100-2242 in the following applications:

  • 1 publication
  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 23760504).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNF20 Antibody

  • BRE1 E3 ubiquitin ligase homolog
  • BRE1
  • BRE1-A
  • BRE1AMGC129667
  • E3 ubiquitin-protein ligase BRE1A
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ11189
  • FLJ20382
  • hBRE1MGC129668
  • homolog of S. cerevisiae BRE1
  • KAIA2779
  • ring finger protein 20KIAA2779

Background

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription. Alternate names for RNF20 include E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, FLJ11189, FLJ20382, KIAA2779, MGC129667, and MGC129668.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNF20 Antibody (NB100-2242)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ChIP, WB.


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ChIP
(1)
WB
(6)
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(6)
Mouse
(1)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-2242 Applications Species
Chen S, Jing Y, Kang X et al. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy. Nucleic Acids Res 2017 Feb 17 [PMID: 28180298]
Chen S, Jing Y, Kang X et al. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy. Nucleic Acids Res. Oct 26 2016 [PMID: 27789696] (WB, Human) WB Human
Wu Y, Chen P, Jing Y et al. Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination PLoS ONE. 2015 Jul 16 [PMID: 26177367] (WB, Human) WB Human
Sussman RT, Stanek TJ, Esteso P et al. The epigenetic modifier Ubiquitin Specific Protease 22 (Usp22) regulates embryonic stem cell differentiation via transcriptional repression of Sex determining region Y-box 2 (Sox2). J Biol Chem 2013 Jun 12 [PMID: 23760504] (WB, Mouse) WB Mouse
Wang E, Kawaoka S, Yu M et al. Histone H2B ubiquitin ligase RNF20 is required for MLL-rearranged leukemia. Proc Natl Acad Sci U S A 2013 Mar 5 [PMID: 23412334] (WB, Human) WB Human
Arora M, Zhang J, Heine GF et al. Promoters active in interphase are bookmarked during mitosis by ubiquitination Nucleic Acids Res 2012 Aug 31 [PMID: 22941662] (WB, Human) WB Human
Jaaskelainen T, Makkonen H, Visakorpi T et al. Histone H2B ubiquitin ligases RNF20 and RNF40 in androgen signaling and prostate cancer cell growth. Mol Cell Endocrinol 2012 Mar [PMID: 22155569]
Hahn MA, Dickson KA, Jackson S et al. The tumor suppressor CDC73 interacts with the ring finger proteins RNF20 and RNF40 and is required for the maintenance of histone 2B monoubiquitination. Hum Mol Genet 2012 Feb [PMID: 22021426]
Taniue K, Oda T, Hayashi T et al. A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis. EMBO Rep 2011 Jul 1 [PMID: 21617703] (ChIP, Human) ChIP Human
Kim J, Roeder RG. Nucleosomal H2B ubiquitylation with purified factors. Methods 2011 Jul [PMID: 21443952]
Show All 13 Publications.

Review for RNF20 Antibody (NB100-2242) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2242:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot RNF20 NB100-2242
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4
reviewed by:
Verified Customer
WB Human 05/16/2012
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotA1

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Verified Customer
05/16/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol RNF20
Entrez
Uniprot