![Immunoprecipitation: DOT1L Antibody [NB100-40846] - Detection of Human DOT1L by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody NB100-40846 used for IP at 3 mcg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti- DOT1L antibody NB100-40845, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time 10 seconds.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Immunoprecipitation-NB100-40846-img0002.jpg "Immunoprecipitation: DOT1L Antibody [NB100-40846] - Detection of Human DOT1L by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody NB100-40846 used for IP at 3 mcg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti- DOT1L antibody NB100-40845, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time 10 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit DOT1L Antibody - BSA Free (NB100-40846) is a polyclonal antibody validated for use in IP. Anti-DOT1L Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue1485 and the C-terminus (residue 1537) of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DOT1L |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-40846 | Applications | Species |
|---|---|---|
| Vernimmen D, Lynch MD, De Gobbi M et al. Polycomb eviction as a new distant enhancer function. Genes Dev 2011-08-01 [PMID: 21828268] |
Secondary Antibodies |
Isotype Controls |
Research Areas for DOT1L Antibody (NB100-40846)Find related products by research area.
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| Gene Symbol | DOT1L |
| Entrez |
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| Uniprot |
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