Dnmt3L Antibody

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Western Blot: Dnmt3L Antibody [NBP2-27098] - Analysis of Dnmt3L using Dnmt3L antibody. GST tagged full length recombinant human Dnmt3L probed with 1) prebleed and 2) Dnmt3L antibody at 2 ug/ml. Goat anti-rabbit Ig HRP ...read more
Immunohistochemistry-Paraffin: Dnmt3L Antibody [NBP2-27098] - Analysis of Dnmt3L in human kidney using Dnmt3L antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Pm, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Dnmt3L Antibody Summary

Immunogen
A portion of amino acids 130-180 from human Dnmt3L was used as the immunogen for this antibody.
Predicted Species
Rhesus Macaque (94%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNMT3L
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4ug/ml~
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24529261).
Publications
Read Publication using
NBP2-27098 in the following applications:

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 94% in Macaque, and White-cheeked Gibbon, 89% in Bovine, New World Monkey, Chinese Hamster, Equine, Rat, and Porcine, and 83% in Canine, and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for Dnmt3L Antibody

  • DNA (cytosine-5-)-methyltransferase 3-like
  • DNA (cytosine-5)-methyltransferase 3-like
  • human cytosine-5-methyltransferase 3-like protein, 10cytosine-5-methyltransferase 3-like protein
  • MGC1090

Background

DNMT3L is a nuclear DNA methyltransferase (DNMT)-like protein. DNMTs are a family of enzymes that catalyze the transfer of a methyl group to DNA, a covalent genomic modification mechanism has a wide range of biological functions. DNMTs are most well recognized for regulating and maintaining genomic imprinting, a germline-specific epigenetic genome process involving both DNA methylation and histone modifications. Imprinting results in certain genes being expressed in a parent-of-origin manner and is essential for normal development. Three active DNMTs have been identified in mammals, DNMT1, DNMT3A and DNMT3B. However, DNMT3L lacks the amino acid residues necessary for methyltransferase activity and is catalytically inactive. Hence, DNMT3L cannot directly methylate DNA. Rather, DNMT3L is thought to function as a regulator of DNA methylation. For example, DNMT3L co-localizes with DNMT3a and DNMT3b in the nucleus and stimulates their activity through protein-protein interactions possibly by anchoring the DNMTs to the chromatin. DNMT3L is thought to be required for maternal genomic imprints and there is evidence that DNMT3L also mediates transcriptional repression through interaction with histone deacetylase 1. Dysregulation of DNMT3L has been associated with cancer, and hence DNMT3L may play an important role in aberrant gene expression during carcinogenesis through alteration of methylation patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dnmt3L Antibody (NBP2-27098)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Diseases for Dnmt3L Antibody (NBP2-27098)

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Blogs on Dnmt3L.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol DNMT3L
Uniprot