HAF016). Specific bands were detected for DNMT3B at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, BG01V human embryonic stem cells, and human thymus tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human DNMT3B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7646) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for DNMT3B at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7646-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85815-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27699-50ul | |||
![Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunohistochemistry-Paraffin-NB300-516-img0010.jpg)
![Western Blot: DNMT3B Antibody [NB300-516] - Homogenized mouse brain cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Western-Blot-NB300-516-img0006.jpg)
![Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunohistochemistry-Paraffin-NB300-516-img0008.jpg)
![Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunohistochemistry-Paraffin-NB300-516-img0009.jpg)
![Simple Western: DNMT3B Antibody [NB300-516] - Simple Western lane view shows a specific band for DNMT3B in 0.5 mg/mL of BG01V lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Simple-Western-NB300-516-img0007.jpg)
![Simple Western: DNMT3B Antibody [NB300-516] - Analysis of 25 ug of HeLa cell lysates and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Simple-Western-NB300-516-img0011.jpg)
![Western Blot: DNMT3B Antibody [NB300-516] - DNMT3B is a direct target of miR-30a & miR-379. a The effect of miRNA mimics (PM-30a or PM-379, 20 nM) on the luciferase activities of the constructs containing the wild-type (wt-3′-UTR) or mutant-type 3′-UTR (mt-30a-3′-UTR or mt-379-3′-UTR) in OEC-M1 cells. The relative luciferase activity of each sample is measured at 48 h after transfection & normalized to Renilla luciferase activity. The data are represented as mean ± SD; ***p < 0.001 versus control mimics (PM-NC). b RT-PCR & Western blot analysis of DNMT3B level in SCC-15 & OEC-M1 cells after treatment with control mimics (NC), or miRNA mimics (PM-30a or PM-379, 20 nM) or miRNA inhibitor (AM-30a or AM-379, 20 nM) for 48 h. GAPDH & alpha -tubulin were used as internal control, respectively. c RT-PCR analysis of ADHFE1 & ALDH1A2 expression level after treatment with control mimics (NC, 20 nM), or miRNA mimics (PM-30a or PM-379, 20 nM) for 48 h in SCC-15 & OEC-M1 cells. GAPDH was used as internal control. d Relative miR-30a & miR-379 expression levels in 33 of adjacent normal tissues (N) compared with their own tumors (T). e Correlation analysis of DNMT3B & miR-30a or miR-379 in OSCC patients (n = 33) by qRT-PCR analysis. Pearson correlation coefficients & p-values were calculated as indicated. Red, tumor part; green, normal part Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-516_rabbit-polyclonal-dnmt3b-antibody-310202416205125.jpg)
![Western Blot: DNMT3B Antibody [NB300-516] - Arecoline & NNK induced DNMT3B activity & repressed ADHFE1, ALDH1A2 & miRNAs expression. a qRT–PCR analysis of miR-30a & miR-379 expression level after treatment with arecoline (50 μM) or NNK (10 μM) for indicated days. b RT-PCR analysis of ADHFE1, ALDH1A2 level & western blot analysis of DNMT3B level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) for indicated times. GAPDH & alpha -tubulin were used as internal control. c ChIP assay of ADHFE1 & ALDH1A2 promoter region was performed with DOK cells using anti-DNMT3B antibody after treatment with vehicle control (DMSO, 10 nM), arecoline (50 μM) or NNK (10 μM) for 5 days. Mouse IgG (mIgG) antibody was used as negative control. d RT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) alone or combined with 5-aza-dC (5 μM) for 5 days. GAPDH was used as internal control. e qRT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with vehicle control (C), or arecoline (50 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM), or NNK (10 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM) for 5 days. GAPDH was used as an internal control. All data are presented as mean ± SD; **p < 0.01; ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-516_rabbit-polyclonal-dnmt3b-antibody-310202416212361.jpg)
![Western Blot: DNMT3B Antibody [NB300-516] - Arecoline & NNK induced DNMT3B activity & repressed ADHFE1, ALDH1A2 & miRNAs expression. a qRT–PCR analysis of miR-30a & miR-379 expression level after treatment with arecoline (50 μM) or NNK (10 μM) for indicated days. b RT-PCR analysis of ADHFE1, ALDH1A2 level & western blot analysis of DNMT3B level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) for indicated times. GAPDH & alpha -tubulin were used as internal control. c ChIP assay of ADHFE1 & ALDH1A2 promoter region was performed with DOK cells using anti-DNMT3B antibody after treatment with vehicle control (DMSO, 10 nM), arecoline (50 μM) or NNK (10 μM) for 5 days. Mouse IgG (mIgG) antibody was used as negative control. d RT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) alone or combined with 5-aza-dC (5 μM) for 5 days. GAPDH was used as internal control. e qRT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with vehicle control (C), or arecoline (50 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM), or NNK (10 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM) for 5 days. GAPDH was used as an internal control. All data are presented as mean ± SD; **p < 0.01; ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-516_rabbit-polyclonal-dnmt3b-antibody-31020241621836.jpg)
NL012). SSEA-4 was detected using Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # 
HAF016). A specific band was detected for DNMT3B at approximately 100-110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # ![Immunocytochemistry/Immunofluorescence: DNMT3B Antibody [NBP1-85815] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85815-img0007.jpg "Immunocytochemistry/Immunofluorescence: DNMT3B Antibody [NBP1-85815] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.")
![Western Blot: DNMT3B Antibody (SY09-07) [NBP2-67398] - Analysis of Dnmt3b on different lysates using anti-Dnmt3b antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: A431](http://images.novusbio.com/fullsize/DNMT3B-Antibody-SY09-07-Western-Blot-NBP2-67398-img0001.jpg "Western Blot: DNMT3B Antibody (SY09-07) [NBP2-67398] - Analysis of Dnmt3b on different lysates using anti-Dnmt3b antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: A431")
![Western blot analysis of NCCIT cell lysates with NBP2-67398 at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 96 kDa Observed band size: 81 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67398 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67398_-western-blot-639204586668650747.jpg)
![Western Blot: DNMT3B Antibody [NBP2-59291] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Western-Blot-NBP2-59291-img0001.jpg "Western Blot: DNMT3B Antibody [NBP2-59291] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: DNMT3B Antibody [NBP2-59291] - Human HeLa cells were stained with the antibody against DNMT3B and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the DNMT3B antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunofluorescence-NBP2-59291-img0003.jpg)
![ELISA: DNMT3B Antibody [NBP2-59291] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody directed against DNMT3B. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:220,000.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-ELISA-NBP2-59291-img0002.jpg)
![Western Blot: DNMT3B Antibody (SR2574) [NBP3-27699] - Western blot analysis of (1) A549 cell lysate; (2) A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27699_rabbit-dnmt3b-mab-sr2574-75202410133445.jpg "Western Blot: DNMT3B Antibody (SR2574) [NBP3-27699] - Western blot analysis of (1) A549 cell lysate; (2) A431 cell lysate.")
![Western Blot: DNMT3B Antibody [NBP3-18646] - Figure 2. Western blot analysis using the antibody directed against DNMT3B Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Western-Blot-NBP3-18646-img0001.jpg "Western Blot: DNMT3B Antibody [NBP3-18646] - Figure 2. Western blot analysis using the antibody directed against DNMT3B Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left.")
![ELISA: DNMT3B Antibody [NBP3-18646] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse DNMT3B in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,500.](http://images.novusbio.com/fullsize/DNMT3B Antibody-ELISA-NBP3-18646-img0002.jpg)
![Western Blot: Dnmt3b Overexpression Lysate (Adult Normal) [NBP2-07562] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dnmt3b.](http://images.novusbio.com/fullsize/Dnmt3b-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07562-img0001.jpg "Western Blot: Dnmt3b Overexpression Lysate (Adult Normal) [NBP2-07562] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dnmt3b.")
![ELISA: Human DNMT3B - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31444] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31444_human-dnmt3b-ready-to-use-elisa-kit-colorimetric-20620241533998.png "ELISA: Human DNMT3B - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31444] - Standard Curve Reference")
![ELISA: Human DNMT3B ELISA Kit (Colorimetric) [NBP3-31443] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31443_human-dnmt3b-elisa-kit-colorimetric-206202415273786.png "ELISA: Human DNMT3B ELISA Kit (Colorimetric) [NBP3-31443] - Standard Curve Reference")
