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Dnmt2 Antibody (102B1259.2)

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Western Blot: Dnmt2 Antibody (102B1259.2) [NB200-587] - Analysis of Dnmt2 in transfected cell lysate using Dnmt2 monoclonal antibody at 2 ug/ml.
Western Blot: Dnmt2 Antibody (102B1259.2) [NB200-587] - Analysis of Dnmt2 in transfected cell lysate using Dnmt2 monoclonal antibody at 2 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
102B1259.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB200-587AF405
Alexa Fluor 488
NB200-587AF488
Alexa Fluor 647
NB200-587AF647
Alexa Fluor 700
NB200-587AF700
Allophycocyanin
NB200-587APC
Biotin
NB200-587B
DyLight 350
NB200-587UV
DyLight 405
NB200-587V
DyLight 488
NB200-587G
DyLight 550
NB200-587R
DyLight 650
NB200-587C
DyLight 680
NB200-587FR
DyLight 755
NB200-587IR
FITC
NB200-587F
HRP
NB200-587H
PE
NB200-587PE
PerCP
NB200-587PCP
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Dnmt2 Antibody (102B1259.2) Summary

Immunogen
This antibody was raised against synthetic peptides corresponding to mouse Dnmt2.
Localization
Nuclear
Specificity
In mouse Dnmt2 transfected cell lysate, a 42 kDa band is oberseved.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TRDMT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Publications
Read Publications using
NB200-587 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Dnmt2 Antibody (102B1259.2)

  • DMNT2
  • DNA (cytosine-5-)-methyltransferase 2
  • DNA (cytosine-5)-methyltransferase-like protein 2
  • DNA methyltransferase homolog HsaIIP
  • DNA methyltransferase-2
  • DNA MTase homolog HsaIIP
  • DNMT2
  • EC 2.1.1
  • EC 2.1.1.29
  • M.HsaIIP
  • MHSAIIP
  • PUMET
  • RNMT1
  • tRNA (cytosine-5-)-methyltransferase
  • tRNA aspartic acid methyltransferase 1

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was probed with 2 ug/ml anti-Dnmt3a in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the decrease in Dnmt3a expression upon treatment with 500 uM Zebularine (arrowhead). Additional bands at 90 and 75 kDa can also be detected with this antibody and may represent alternative splice variants. Tubulin is shown as a loading control
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H00004502-M01
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Dnmt2 Antibody
(102B1259.2)
NB200-587
Dnmt2 Antibody (102B1259.2)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
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Publications for Dnmt2 Antibody (NB200-587)(2)

We have publications tested in 2 confirmed species: Mouse, Zebrafish.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
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Secondary Antibodies

 

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Other Available Formats

Alexa Fluor 405 Labeled NB200-587AF405
Alexa Fluor 488 Labeled NB200-587AF488
Alexa Fluor 647 Labeled NB200-587AF647
Alexa Fluor 700 Labeled NB200-587AF700
Allophycocyanin Labeled NB200-587APC
Biotin Labeled NB200-587B
DyLight 350 Labeled NB200-587UV
DyLight 405 Labeled NB200-587V
DyLight 488 Labeled NB200-587G
DyLight 550 Labeled NB200-587R
DyLight 650 Labeled NB200-587C
DyLight 680 Labeled NB200-587FR
DyLight 755 Labeled NB200-587IR
HRP Labeled NB200-587H
PE Labeled NB200-587PE
PerCP Labeled NB200-587PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol TRDMT1
Entrez
Uniprot