Dnmt2 Antibody (102B1259.2)

Western Blot: Dnmt2 Antibody (102B1259.2) [NB200-587] - Analysis of Dnmt2 in transfected cell lysate using Dnmt2 monoclonal antibody at 2 ug/ml.

Flow Cytometry: Dnmt2 Antibody (102B1259.2) [NB200-587] - An intracellular stain was performed on Jurkat cells with Dnmt2 Antibody [102B1259.2] NB200-587 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

• Reactivity: Hu, Mu, Ze
• Applications: WB, Flow
• Clone: 102B1259.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Dnmt2 Antibody (102B1259.2) Summary

• Immunogen: This antibody was raised against synthetic peptides corresponding to mouse Dnmt2.
• Localization: Nuclear
• Specificity: In mouse Dnmt2 transfected cell lysate, a 42 kDa band is oberseved.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRDMT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-5 ug/ml
  • Western Blot 1:100-1:2000

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 17289917).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Dnmt2 Antibody (102B1259.2)

• DMNT2
• DNA (cytosine-5-)-methyltransferase 2
• DNA (cytosine-5)-methyltransferase-like protein 2
• DNA methyltransferase homolog HsaIIP
• DNA methyltransferase-2
• DNA MTase homolog HsaIIP
• DNMT2
• EC 2.1.1
• EC 2.1.1.29
• M.HsaIIP
• MHSAIIP
• PUMET
• RNMT1
• tRNA (cytosine-5-)-methyltransferase
• tRNA aspartic acid methyltransferase 1

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dnmt2 Antibody (NB200-587)(2)

Publications using NB200-587

• Su ZY, Zhang C, Lee JH et al. Requirement and Epigenetics Reprogramming of Nrf2 in Suppression of Tumor Promoter TPA-Induced Mouse Skin Cell Transformation by Sulforaphane. Cancer Prev Res (Phila). 2014 Mar 1 [PMID: 24441674] (WB, Mouse)
  
  Details:
  JB6 P+ mouse epidermal cells, Fig 6.
• Rai K, Chidester S, Zavala CV et al. Dnmt2 functions in the cytoplasm to promote liver, brain, and retina development in zebrafish. Genes Dev. 2007 Feb 1 [PMID: 17289917] (WB, Zebrafish)
  
  Details:
  WB (zebrafish embryos), Fig. 2.

Review for Dnmt2 Antibody (NB200-587) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Lindsey Johnson Immunocytochemistry Human 08/10/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: HeLa cells
• Species: Human
• Lot: 102B1259.2

Bioinformatics

• Gene Symbol: TRDMT1
• Entrez:
  • 1787(Human)
  • 13434(Mouse)
• Uniprot:
  • Q6ICS7(Human)